| Human angiogenin presents neuroprotective and migration effects in neuroblastoma cells. | |
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MedLine Citation:
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PMID: 20174961 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human angiogenin (ANG) has been highlighted as an angiogenic factor which supports primary and metastatic tumor growth. Recent genetic studies have shown that ANG is presented as a susceptibility gene for amyotrophic lateral sclerosis (ALS) and ALS-frontotemporal dementia (ALS-FTD). They found several missense mutations, including K40I, which present the weakest functional activity in ANG variants. In this study, we investigate whether human wild type ANG (wANG) and its variant K40I (mANG) maintain their divergent functional capacities in neuronal cells. To evaluate this, SH-SY5Y neuroblastoma cells were transfected with wANG and mANG DNA and identified both wild and mutant ANG are localized to nuclei and have no effects on proliferation. We have shown that human wANG prevented cell death under H(2)O(2)-induced oxidative stress in both SH-SY5Y and NSC-34 cells, tested by MTT assay. These effects were more enhanced in motor neuron cell NSC-34. wANG also played a role in cell migration, while mANG decreased these functional activities. Immunoblot analysis revealed that the intracellular signaling of ERK1/2 (at Thr183/Tyr185) was increased following transfection of the wANG gene, and significantly decreased by mANG in neuronal cells. These findings suggest that human ANG plays a critical role in cell protection and migration following alterations in ERK1/2 signaling in SH-SY5Y cells. This may provide the possible relationship between mutations in hANG and other neurodegenerative diseases as well as ALS. |
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Authors:
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Goang-Won Cho; Byung Yong Kang; Seung Hyun Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-20 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 340 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-15 Completed Date: 2010-09-21 Revised Date: 2011-07-19 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 133-41 Citation Subset: IM |
Affiliation:
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Department of Neurology, College of Medicine, Hanyang University, #17 Haengdang-dong, Seongdong-gu, Seoul 139-791, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Death Cell Line, Tumor Cell Movement* Cell Nucleus / enzymology Cell Proliferation Cytoprotection* Dose-Response Relationship, Drug Humans Hydrogen Peroxide / pharmacology Mice Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Motor Neurons / drug effects, enzymology*, pathology Mutation Nerve Degeneration / enzymology, genetics, pathology, prevention & control* Neuroblastoma / enzymology*, genetics, pathology Oxidants / pharmacology Oxidative Stress Ribonuclease, Pancreatic / genetics, metabolism* Signal Transduction Transfection |
| Chemical | |
Reg. No./Substance:
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0/Oxidants; 7722-84-1/Hydrogen Peroxide; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 3.1.27.-/angiogenin; EC 3.1.27.5/Ribonuclease, Pancreatic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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