Document Detail


Human alpha3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-4R determinant at the nonreducing terminus.
MedLine Citation:
PMID:  11320059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human alpha3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Galbeta1-4(Fucalpha1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAcbeta1-4GlcNAc, generating GalNAcbeta1-4(Fucalpha1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2-4 GlcNAc units efficiently to products containing a GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme.
Authors:
J Natunen; O Aitio; J Helin; H Maaheimo; R Niemelä; S Heikkinen; O Renkonen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycobiology     Volume:  11     ISSN:  0959-6658     ISO Abbreviation:  Glycobiology     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-04-26     Completed Date:  2001-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9104124     Medline TA:  Glycobiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  209-16     Citation Subset:  IM    
Affiliation:
Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Acetylglucosamine / metabolism*
Carbohydrate Sequence
Chitin / metabolism*
Fucosyltransferases / metabolism*
Humans
Molecular Sequence Data
Oligosaccharides / chemistry,  metabolism*
Spectrometry, Mass, Electrospray Ionization
Chemical
Reg. No./Substance:
0/Oligosaccharides; 1398-61-4/Chitin; 7512-17-6/Acetylglucosamine; EC 2.4.1.-/Fucosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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