| Human alpha3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-4R determinant at the nonreducing terminus. | |
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MedLine Citation:
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PMID: 11320059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human alpha3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Galbeta1-4(Fucalpha1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAcbeta1-4GlcNAc, generating GalNAcbeta1-4(Fucalpha1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2-4 GlcNAc units efficiently to products containing a GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme. |
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Authors:
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J Natunen; O Aitio; J Helin; H Maaheimo; R Niemelä; S Heikkinen; O Renkonen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glycobiology Volume: 11 ISSN: 0959-6658 ISO Abbreviation: Glycobiology Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-04-26 Completed Date: 2001-07-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9104124 Medline TA: Glycobiology Country: England |
Other Details:
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Languages: eng Pagination: 209-16 Citation Subset: IM |
Affiliation:
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Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylglucosamine
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metabolism* Carbohydrate Sequence Chitin / metabolism* Fucosyltransferases / metabolism* Humans Molecular Sequence Data Oligosaccharides / chemistry, metabolism* Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 1398-61-4/Chitin; 7512-17-6/Acetylglucosamine; EC 2.4.1.-/Fucosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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