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Human Vγ9Vδ2-T cells efficiently kill influenza virus-infected lung alveolar epithelial cells.
MedLine Citation:
PMID:  23353835     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
γδ-T cells play an indispensable role in host defense against different viruses, including influenza A virus. However, whether these cells have cytotoxic activity against influenza virus-infected lung alveolar epithelial cells and subsequently contribute to virus clearance remains unknown. Using influenza virus-infected A549 cells, human lung alveolar epithelial cells, we investigated the cytotoxic activity of aminobisphosphonate pamidronate (PAM)-expanded human Vγ9Vδ2-T cells and their underlying mechanisms. We found that PAM could selectively activate and expand human Vγ9Vδ2-T cells. PAM-expanded human Vγ9Vδ2-T cells efficiently killed influenza virus-infected lung alveolar epithelial cells and inhibited virus replication. The cytotoxic activity of PAM-expanded Vγ9Vδ2-T cells was dependent on cell-to-cell contact and required NKG2D activation. Perforin-granzyme B, tumor-necrosis factor-related apoptosis-inducing ligand (TRAIL) and Fas-Fas ligand (FasL) pathways were involved in their cytotoxicity. Our study suggests that targeting γδ-T cells by PAM can potentially offer an alternative option for the treatment of influenza virus.Cellular & Molecular Immunology advance online publication, 28 January 2013; doi:10.1038/cmi.2012.70.
Authors:
Hong Li; Zheng Xiang; Ting Feng; Jinrong Li; Yinping Liu; Yingying Fan; Qiao Lu; Zhongwei Yin; Meixing Yu; Chongyang Shen; Wenwei Tu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-28
Journal Detail:
Title:  Cellular & molecular immunology     Volume:  -     ISSN:  2042-0226     ISO Abbreviation:  Cell. Mol. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101242872     Medline TA:  Cell Mol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Joint Research Center of West China Second University Hospital of Sichuan University and Department of Paediatrics and Adolescent Medicine of University of Hong Kong, Sichuan University, Chengdu 610041, China.
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