Document Detail


Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus.
MedLine Citation:
PMID:  23150874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GW182 family proteins play important roles in microRNA (miRNA)-mediated gene silencing. They interact with Argonaute (Ago) proteins and localize in processing bodies, which are cytoplasmic foci involved in mRNA degradation and storage. Here, we demonstrated that human GW182 paralog, TNRC6A, is a nuclear-cytoplasmic shuttling protein, and its subcellular localization is conducted by a nuclear export signal (NES) and a nuclear localization signal (NLS) identified in this study. TNRC6A with mutations in its NES region was predominantly localized in the nucleus in an Ago-independent manner. However, it was found that TNRC6A could bring Ago protein into the nucleus via its Ago-interacting motif(s). Furthermore, miRNAs were also colocalized with nuclear TNRC6A-Ago and exhibited gene silencing activity. These results proposed the possibility that TNRC6A plays an important role in navigating Ago protein into the nucleus to lead miRNA-mediated gene silencing.
Authors:
Kenji Nishi; Ai Nishi; Tatsuya Nagasawa; Kumiko Ui-Tei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-13
Journal Detail:
Title:  RNA (New York, N.Y.)     Volume:  19     ISSN:  1469-9001     ISO Abbreviation:  RNA     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-19     Completed Date:  2013-02-21     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  9509184     Medline TA:  RNA     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs
Argonaute Proteins / metabolism*
Autoantigens / genetics,  metabolism*
Cell Nucleus / genetics,  metabolism*
Gene Silencing*
HeLa Cells
Humans
MicroRNAs / metabolism*
Mutation
Nuclear Export Signals / genetics,  physiology
Nuclear Localization Signals / genetics,  metabolism
Nucleocytoplasmic Transport Proteins / metabolism
RNA-Binding Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Argonaute Proteins; 0/Autoantigens; 0/MicroRNAs; 0/Nuclear Export Signals; 0/Nuclear Localization Signals; 0/Nucleocytoplasmic Transport Proteins; 0/RNA-Binding Proteins; 0/autoantigen GW182, human
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