Document Detail


Human T-lymphocyte transformation with human T-cell lymphotropic virus type 2.
MedLine Citation:
PMID:  9420297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human T-cell lymphotrophic virus type 2 (HTLV-2), a common infection of intravenous drug users and subpopulations of Native Americans, is uncommon in the general population. In contrast with the closely related HTLV-1, which is associated with both leukemia and neurologic disorders, HTLV-2 lacks a strong etiologic association with disease. HTLV-2 does shares many properties with HTLV-1, including in vitro lymphocyte transformation capability. To better assess the ability of HTLV-2 to transform lymphocytes, a limiting dilution assay was used to generate clonal, transformed lymphocyte lines. As with HTLV-1, the transformation efficiency of HTLV-2 producer cells was proportionately related to the number of lethally irradiated input cells and was comparable to HTLV-1-mediated transformation efficiency. HTLV-2-infected cells were reproducibly isolated and had markedly increased growth potential compared to uninfected cells; HTLV-2 transformants required the continued presence of exogenous interleukin 2 for growth for several months and were maintained for over 2 years in culture. All HTLV-2-transformed populations were CD2 and/or CD3 positive and B1 negative and were either CD4+ or CD8+ populations or a mixture of CD4+ and CD8+ lymphocytes. Clonality of the HTLV-2 transformants was confirmed by Southern blot analysis of T-cell receptor beta chain rearrangement. Southern blot analysis revealed a range of integrated full-length genomes from one to multiple. In situ hybridization analysis of HTLV-2 integration revealed no obvious chromosomal integration pattern.
Authors:
S L Tarsis; M T Yu; E S Parks; D Persaud; J L Muñoz; W P Parks
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  72     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-01-28     Completed Date:  1998-01-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  841-6     Citation Subset:  IM; X    
Affiliation:
Department of Pediatrics, New York University Medical Center, New York 10016, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Differentiation, T-Lymphocyte / metabolism
Cell Line, Transformed
Cell Transformation, Viral*
Chromosomes, Human / genetics,  virology
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
Human T-lymphotropic virus 2 / genetics,  isolation & purification,  pathogenicity*
Humans
In Situ Hybridization
Phenotype
Proviruses / genetics,  isolation & purification
Reproducibility of Results
T-Lymphocytes / immunology,  virology*
Virology / methods,  statistics & numerical data
Virus Integration / genetics
Chemical
Reg. No./Substance:
0/Antigens, Differentiation, T-Lymphocyte
Comments/Corrections

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