| Human Skeletal Muscle Fiber Type Specific Protein Content. | |
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MedLine Citation:
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PMID: 22469996 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The aim of this project was to develop a method to assess fiber type specific protein content across the continuum of human skeletal muscle fibers. Individual vastus lateralis muscle fibers (n = 264) were clipped into two portions, one for SDS-PAGE fiber typing and one for Western blot protein identification. Following fiber type determination, fiber segments were combined into fiber type specific pools (∼20 fibers/pool) and measured for total protein quantity, GAPDH, citrate synthase, and total p38 content. GAPDH content was 64%, 54%, 160%, and 138% more abundant in MHC I/IIa, MHC IIa, MHC IIa/IIx, and MHC IIx fibers when compared to MHC I. Inversely, citrate synthase content was 528%, 472%, 242%, and 47% more abundant in MHC I, MHC I/IIa, MHC IIa, and MHC IIa/IIx fibers when compared to MHC IIx. Total p38 content was 87% greater in MHC IIa versus MHC I fibers. These data and approach establish a reliable method for human skeletal muscle fiber type specific protein analysis. Initial results show particular proteins exist in a hierarchal fashion throughout the continuum of human skeletal muscle fiber types, further highlighting the necessity of fiber type specific analysis. |
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Authors:
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Andrew J Galpin; Ulrika Raue; Bozena Jemiolo; Todd A Trappe; Matthew P Harber; Kiril Minchev; Scott Trappe |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-30 |
Journal Detail:
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Title: Analytical biochemistry Volume: - ISSN: 1096-0309 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-4-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Human Performance Laboratory, Ball State University, Muncie, IN. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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