Document Detail

Human SP1 but not human AP1 binding to the TGF-beta element in the 5' flanking region of the rat PROalpha1(I) collagen gene.
MedLine Citation:
PMID:  11254109     Owner:  NLM     Status:  MEDLINE    
The consensus TGF-beta element (TGCCCACGGCCAG) located at approximately -161Obp from the start site of transcription of the rat pro alpha1(I) collagen gene has recently been shown to be required for the basal promoter activity of this gene (Meisler et al., J. Cell Biochem. 75: 196, 1999). Site directed mutation of this TGF-beta element resulted in almost complete abolishment of the basal promoter activity of the fibroblasts transfected with the 3.6 ColCat plasmid which contains a 3.6 kb portion of the 5' flanking region of the rat pro alpha1(I) collagen gene linked to the reporter gene, chloramphenicol acetyltransferase (CAT). Southwestern analysis of the nuclear protein binding to the TGF-beta element revealed a 34,000 Da complex while after UV-crosslinking, studies revealed a TGF-beta element nuclear protein complex of 82,000 Da (Ritzenthaler et al., J. Biol. Chem. 268: 13625, 1993). Thus, a multiple protein TGF-beta DNA element complex may exist which may promote the transcription of the rat pro alpha1(I) collagen gene. Since literature findings indicate that a nuclear factor interacts with an SP1-like binding site of the human pro alpha1(I) collagen promoter and an AP-1 binding sequence has been shown to be involved in the regulation of the human pro alpha2(I) collagen gene and both these binding sequences are TGF-beta1 responsive, we determined whether the TGF-beta element located in the 5' flanking region of the rat pro alpha1(I) collagen gene formed complexes with either of these nuclear factors or both.
K R Cutroneo
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular biology reports     Volume:  27     ISSN:  0301-4851     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-03-19     Completed Date:  2001-08-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  191-4     Citation Subset:  IM    
Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405, USA.
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MeSH Terms
5' Untranslated Regions
Bleomycin / pharmacology
Electrophoresis, Polyacrylamide Gel
Fibroblasts / cytology,  drug effects,  metabolism
Genes, Reporter
Procollagen / genetics*
Promoter Regions, Genetic / drug effects,  genetics
Protein Binding
Response Elements / drug effects,  genetics*
Sp1 Transcription Factor / metabolism*,  pharmacology
Transcription Factor AP-1 / metabolism,  pharmacology
Transforming Growth Factors / genetics*,  metabolism,  pharmacology
Grant Support
Reg. No./Substance:
0/5' Untranslated Regions; 0/Procollagen; 0/Sp1 Transcription Factor; 0/Transcription Factor AP-1; 11056-06-7/Bleomycin; 76057-06-2/Transforming Growth Factors

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