| Human RON receptor tyrosine kinase induces complete epithelial-to-mesenchymal transition but causes cellular senescence. | |
| | |
MedLine Citation:
|
PMID: 17588532 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The RON receptor tyrosine kinase is a member of the MET proto-oncogene family and is important for cell proliferation, differentiation, and cancer development. Here, we created a series of Madin-Darby canine kidney (MDCK) epithelial cell clones that express different levels of RON, and have investigated their biological properties. While low levels of RON correlated with little morphological change in MDCK cells, high levels of RON expression constitutively led to morphological scattering or complete and stabilized epithelial-to-mesenchymal transition (EMT). Unexpectedly, MDCK clones expressing higher levels of RON exhibited retarded proliferation and senescence, despite increased motility and invasiveness. RON was constitutively tyrosine-phosphorylated in MDCK cells expressing high levels of RON and undergoing EMT, and the MAPK signaling pathway was activated. This study reveals for the first time that RON alone is sufficient to induce complete and stabilized EMT in MDCK cells, and overexpression of RON does not cause cell transformation but rather induces cell cycle arrest and senescence, leading to impaired cell proliferation. |
| | |
Authors:
|
Marceline Côté; A Dusty Miller; Shan-Lu Liu |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-18 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 360 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-03 Completed Date: 2007-09-05 Revised Date: 2010-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 219-25 Citation Subset: IM |
Affiliation:
|
Department of Microbiology and Immunology, McGill University, Montreal, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Aging / physiology* Cell Differentiation Cell Line Cell Movement Cell Proliferation Dogs Epithelial Cells / cytology*, enzymology* Humans Kidney / cytology, enzymology Mesoderm / cytology*, enzymology* Receptor Protein-Tyrosine Kinases / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
P30 DK047754-14/DK/NIDDK NIH HHS; P30 DK047754-149001/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 2.7.1.-/RON protein; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The nuclear phenotypic plasticity observed in fish during rRNA regulation entails Cajal bodies dynam...
Next Document: Computational modeling and functional analysis of Herpes simplex virus type-1 thymidine kinase and E...