Document Detail


Human Nogo-C overexpression induces HEK293 cell apoptosis via a mechanism that involves JNK-c-Jun pathway.
MedLine Citation:
PMID:  16905119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neurite outgrowth inhibitor protein Nogo-A has been identified as an inhibitor of axonal regeneration, and Nogo-B as a regulator of vasculature remodeling, but the additional roles of Nogo isoforms, especially Nogo-C, have obtained little attention. Nogo-C is weakly expressed in liver and kidney compared to the high expression in skeletal muscle. Here we detected the weak expression of Nogo-C in human embryonic kidney cell line HEK293, and found that Nogo-C expressed in HEK293 could induce cell apoptosis. Further experiments demonstrated the activation of JNK/SAPK and c-Jun, but not p38 in Nogo-C expressed cells. And JNK-specific inhibitor SP600125 could reduce cell apoptosis induced by Nogo-C. Furthermore, the activation of caspase-3 and PARP, the expression and phosphorylation of p53 were also detected. The data first revealed Nogo-C expressed in HEK293 confers apoptosis by inducing caspase-3 and p53 activation through the JNK-c-Jun-dependent pathway.
Authors:
Yicun Chen; Xiaojun Tang; Xiangrong Cao; Huaqun Chen; Xiran Zhang
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Publication Detail:
Type:  Journal Article     Date:  2006-08-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  348     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-22     Completed Date:  2006-10-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  923-8     Citation Subset:  IM    
Affiliation:
The College of Life Science, Nanjing Normal University, The Jiangsu Key Laboratory of Molecular and Medical Biotechnology, Nanjing 210097, PR China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics*
Caspase 3
Caspases / metabolism
Cell Line
Cloning, Molecular
Enzyme Activation / genetics
Humans
JNK Mitogen-Activated Protein Kinases / metabolism,  physiology*
Myelin Proteins / biosynthesis*,  genetics*,  physiology
Protein Isoforms / genetics
Proto-Oncogene Proteins c-jun / physiology*
Signal Transduction / genetics*
Tumor Suppressor Protein p53 / metabolism
Chemical
Reg. No./Substance:
0/Myelin Proteins; 0/Nogo protein; 0/Protein Isoforms; 0/Proto-Oncogene Proteins c-jun; 0/Tumor Suppressor Protein p53; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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