| Human Neutrophil Defensins and Their Effect on Epithelial Cells. | |
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MedLine Citation:
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PMID: 22443519 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: The aims of the present study were 1) to localize human neutrophil defensins (HNP) 1-3 in gingiva and in neutrophil extract-treated epithelial cell monolayers, 2) to determine the effects of HNP-1 on the epithelial cell viability, attachment, and spreading, and 3) to analyze the effect of HNP-1 on the bacterial adherence to epithelial cells. Methods: For localization of HNP 1-3 in gingival tissue and in pre-incubated cell monolayers, immunohistochemical and immunocytochemical methods were used. Viability and proliferation of epithelial cells were determined with commercial kits after incubating the keratinocytes with different HNP-1 concentrations (low: 1-5 μg/ml, moderate: 10 μg/ml, high: 20-50 μg/ml). Attachment and spreading of keratinocytes on fibronectin-coated surfaces in the presence of HNP-1 were evaluated under microscope. Attachment of Fusobacterium nucleatum ATCC 25586 and Prevotella intermedia ATCC 25611 on keratinocytes pre-incubated with HNP-1 were determined with a standard antibiotic test. Results: HNP 1-3 localized in the junctional epithelium of clinically healthy gingiva and in the pocket epithelium of gingiva with periodontitis. When keratinocyte monolayers were incubated with neutrophil extracts, HNP 1-3 were bound to the periphery of the growing cell colonies. In low HNP-1 concentrations, the keratinocyte proliferation enhanced. Moderate and high concentrations of HNP-1 increased the cellular death significantly. HNP-1 increased the attachment and spreading of keratinocytes on fibronectin-coated surfaces, and bacterial attachment to keratinocytes, in a concentration-dependent manner. Conclusions: HNP-1 plays a role in the integrity of keratinocytes by stimulating their proliferation, attachment, and spreading, while higher doses increase the bacterial attachment and keratinocyte death. |
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Authors:
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Ulvi K Gursoy; Eija Könönen; Noora Luukkonen; Veli-Jukka Uitto |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-23 |
Journal Detail:
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Title: Journal of periodontology Volume: - ISSN: 1943-3670 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Periodontology, Institute of Dentistry, University of Turku, FI-20520, Turku, Finland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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