Document Detail


Human NT2 neural precursor-derived tumor-infiltrating cells as delivery vehicles for treatment of glioblastoma.
MedLine Citation:
PMID:  20113165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neural stem cells used as a cellular vehicle have been proven effective in targeting glioblastomas in animal models. In an attempt to identify human sources of such cellular vehicles other than human brain tissue, we tested whether the well-established human NT2 cell line, which shares many characteristics of neural precursor cells, could be used to derive new cellular vehicles with glioma tropism. After treating NT2 cells with retinoic acid for 2 weeks, we isolated, using Boyden chambers, a group of NT2 cells that migrated toward human U87 glioblastoma cells. In mice, these cells could home in on intracranial U87 glioblastoma xenografts after systemic administration via the tail vein. To test the feasibility of using these tumor-infiltrating cells for targeted glioma therapy, we injected them, after introducing the herpes simplex virus thymidine kinase gene into the cells, into the brain side contralateral to a site pre-inoculated with U87 cells. After ganciclovir injection, we observed inhibition of tumor growth and significantly prolonged survival of tumor-inoculated animals. NT2 cells are stable, easy to cultivate, and amenable to scale-up for cell production, and thus our method holds promise for generating a human cell-based delivery vehicle for clinical cancer therapy.
Authors:
Ying Zhao; Shu Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human gene therapy     Volume:  21     ISSN:  1557-7422     ISO Abbreviation:  Hum. Gene Ther.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9008950     Medline TA:  Hum Gene Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  683-94     Citation Subset:  IM    
Affiliation:
Institute of Bioengineering and Nanotechnology, Singapore 138669.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism,  pathology
Brain Neoplasms / metabolism,  pathology,  therapy
Ganciclovir / therapeutic use
Glioblastoma / drug therapy,  pathology,  therapy*
Glioma / metabolism,  pathology,  therapy
Humans
Male
Mice
Mice, Nude
Nervous System
Nervous System Neoplasms / drug therapy,  therapy
Neurons / metabolism,  pathology
Vehicles
Chemical
Reg. No./Substance:
0/Vehicles; 82410-32-0/Ganciclovir

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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