Document Detail


Human immunodeficiency virus-1 inhibition of immunoamphisomes in dendritic cells impairs early innate and adaptive immune responses.
MedLine Citation:
PMID:  20451412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendritic cells (DCs) in mucosal surfaces are early targets for human immunodeficiency virus-1 (HIV-1). DCs mount rapid and robust immune responses upon pathogen encounter. However, immune response in the early events of HIV-1 transmission appears limited, suggesting that HIV-1 evade early immune control by DCs. We report that HIV-1 induces a rapid shutdown of autophagy and immunoamphisomes in DCs. HIV-1 envelope activated the mammalian target of rapamycin pathway in DCs, leading to autophagy exhaustion. HIV-1-induced inhibition of autophagy in DC increased cell-associated HIV-1 and transfer of HIV-1 infection to CD4(+) T cells. HIV-1-mediated downregulation of autophagy in DCs impaired innate and adaptive immune responses. Immunoamphisomes in DCs engulf incoming pathogens and appear to amplify pathogen degradation as well as Toll-like receptor responses and antigen presentation. The findings that HIV-1 downregulates autophagy and impedes immune functions of DCs represent a pathogenesis mechanism that can be pharmacologically countered with therapeutic and prophylactic implications.
Authors:
Fabien P Blanchet; Arnaud Moris; Damjan S Nikolic; Martin Lehmann; Sylvain Cardinaud; Romaine Stalder; Eduardo Garcia; Christina Dinkins; Florence Leuba; Li Wu; Olivier Schwartz; Vojo Deretic; Vincent Piguet
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-05-06
Journal Detail:
Title:  Immunity     Volume:  32     ISSN:  1097-4180     ISO Abbreviation:  Immunity     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-06-21     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  9432918     Medline TA:  Immunity     Country:  United States    
Other Details:
Languages:  eng     Pagination:  654-69     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Dermatology, University Hospital and Medical School of Geneva, 1211 Geneva, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Adaptive Immunity*
Autophagy
Base Sequence
CD4-Positive T-Lymphocytes / virology
Cells, Cultured
Dendritic Cells / immunology*,  pathology,  virology*
Down-Regulation
Flow Cytometry
HIV Infections / immunology*
HIV-1 / physiology*
Humans
Immunity, Innate*
Immunoblotting
Intracellular Signaling Peptides and Proteins / metabolism
Lysosomes / immunology,  virology
Molecular Sequence Data
Phagosomes / immunology*,  virology
Protein-Serine-Threonine Kinases / metabolism
Ribosomal Protein S6 Kinases, 70-kDa / metabolism
Signal Transduction
TOR Serine-Threonine Kinases
Grant Support
ID/Acronym/Agency:
R01 AI068493-05/AI/NIAID NIH HHS; R01 AI068493-06/AI/NIAID NIH HHS; R01-AI068493/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Intracellular Signaling Peptides and Proteins; EC 2.7.1.1/MTOR protein, human; EC 2.7.1.1/TOR Serine-Threonine Kinases; EC 2.7.1.37/ribosomal protein S6 kinase, 70kD, polypeptide 1; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Ribosomal Protein S6 Kinases, 70-kDa
Comments/Corrections
Comment In:
Immunity. 2010 May 28;32(5):587-90   [PMID:  20510868 ]

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