Document Detail


Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
MedLine Citation:
PMID:  20929355     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Early germ-like cells (GLCs) derived from human embryonic stem cells (hESCs) have presented new opportunities to study germ cell differentiation in vitro. However, differentiation conditions that facilitate the formation of haploid cells from the derived GLCs have eluded the field. The inability to propagate GLCs in culture is a further limitation, resulting in inconsistent rederivations of GLCs from hESCs with relatively few GLCs in these heterogeneous populations. Here we found in vitro conditions that enrich for DDX4/POU5F1+ GLCs (∼60%) and that has enabled continual propagation for >50 passages without loss of phenotype. Clonal isolation of single GLCs from these mixed cultures generated 3 GLC (>90% DDX4/POU5F1+) and 2 hESC (<0.1% DDX4+) lines that could be continually expanded without loss of phenotype. Differentiation of clonal GLC lines in serum resulted in expression of postmeiotic markers and >11% were haploid, ∼5-fold higher than previous studies. The robust clonal meiotic competent and incompetent GLC lines will be used to understand the factors controlling human germ cell meiosis and postmeiotic maturation.
Authors:
Franklin D West; Jennifer L Mumaw; Amalia Gallegos-Cardenas; Amber Young; Steven L Stice
Related Documents :
2995655 - Cyclic nucleotide phosphodiesterases in somatic and germ cells of mouse seminiferous tu...
9853835 - Developmental fates of the mouse germ cell line.
6166835 - Effects of a bacteriocin from mycobacterium smegmatis on balb/3t3 and simian virus 40-t...
115745 - Hybrid dysgenesis in drosophila melanogaster: the biology of female and male sterility.
15784465 - Synergistic inhibition of tumor necrosis factor-related apoptosis-inducing ligand-induc...
10669935 - Immunolocalization of androgen receptors in testicular cells during postnatal developme...
Publication Detail:
Type:  Journal Article     Date:  2010-12-02
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1079-88     Citation Subset:  IM    
Affiliation:
1 Regenerative Bioscience Center, University of Georgia , Athens, Georgia .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A 2009 varicella outbreak in a Connecticut residential facility for adults with intellectual disabil...
Next Document:  Transfusion-transmitted malaria in countries where malaria is endemic: a review of the literature fr...