Document Detail

Human HT-29 colon carcinoma cells: mucin production and tumorigenicity in relation to growth phases.
MedLine Citation:
PMID:  8572596     Owner:  NLM     Status:  MEDLINE    
The main aim of this study was to determine whether changes in mucin production/secretion during the growth phases of a human adenocarcinoma cell line, HT-29, are associated with a different tumorigenic potential. HT-29 cells cultured in DMEM supplemented with 10% FCS were harvested in the exponential and post-confluent phases of growth. Metabolic labeling of the cells was performed using [3H]-glucosamine. Following a 24 hr-incubation period, radioactivity was measured in 1 ml-aliquots of cell cytosol and culture medium. Concurrently, mucin synthesis was assessed by size exclusion chromatography of [3H]-glucosamine-labeled glycoprotein in a Sepharose CL-4B column. Clonigenic assay in soft agar of pre- and post-confluent HT-29 cells was determined by scoring viable colonies according to size and number using phase-contrast microscopy. To assess in vivo tumorigenicity, pre-and post-confluent HT-29 cells (4 x 10(6)) in 0.2 ml DMEM were inoculated into nude mice. Tumor size and volume were recorded for 31 days. H-29 cells grew as multilayers of unpolarized, undifferentiated cells. Colony forming efficiency was similar at all growth stages. A significant increase in mucin synthesis was noted in HT-29 cells harvested in the exponential phase of growth compared to the stationary phase (148.3 +/- 41.9 versus 49.1 +/- 5.0, mean +/- SE, dpm/4 x 10(3) cells, p < 0.05). Mucin secretion was not significantly changed. Tumorigenicity in nude mice was consistently higher when the cells were injected in the exponential phase of growth. On day 31 after cell inoculation the average tumor volume/site was 332.7 mm3 in mice injected with HT-29 cells in log phase compared to 142.7 mm3 (p < 0.01) in animals which received post-confluent cells. Tumor weights were 0.36 g and 0.22 g respectively (p < 0.05). The present results indicate a definitive correlation between growth phases of HT-29 cells and mucin synthesis: mucin production was significantly higher in exponentially growing cells. The cloning efficiency in soft agar, a marker of in vitro tumorigenicity, was similar in HT-29 cells irrespective of the growth stage. A main finding of the present study was the increased in vivo tumorigenicity of colon cancer cells inoculated into athymic mice in the log phase of growth compared to cells harvested at post-confluence. These results are consistent with the view that mucin plays an important contributory role in determining tumorigenicity.
Y Niv; B Schwartz; Y Amsalem; S A Lamprecht
Related Documents :
21874456 - An in vitro system to study sertoli cell blood-testis barrier dynamics.
22288076 - A strong two-photon induced phosphorescent golgi-specific in vitro marker based on a he...
22680506 - Duration of microbead seeding on endothelial cells significantly affects their response...
12631706 - Loss of protooncogene c-myc function impedes g1 phase progression both before and after...
19549816 - Tunable interplay between epidermal growth factor and cell-cell contact governs the spa...
12789276 - Development of melanocyte precursors from the vertebrate neural crest.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  15     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1995 Sep-Oct
Date Detail:
Created Date:  1996-03-01     Completed Date:  1996-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  2023-7     Citation Subset:  IM    
Gastroenterology Department, Soroka Medical Center, Beer-Sheva, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Division
Colonic Neoplasms / metabolism,  pathology*
Mice, Nude
Mucins / biosynthesis*
Tumor Cells, Cultured
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of C18 fatty acid isomers on DNA synthesis in hepatoma and breast cancer cells.
Next Document:  Luteinizing hormone releasing hormone, estrogen, and progesterone receptors in canine mammary lesion...