Document Detail


Human fibroblasts for large-scale "omics" investigations of ATM gene function.
MedLine Citation:
PMID:  21901628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ATM (gene mutated in ataxia-telangiectasia) is a critical central component of the pleiotropic responses of cells to ionizing radiation-induced stress. To gain insight into molecular mechanisms and to enhance our understanding of ATM functions, we have advanced a human model cell system, derived from genetically defined immortal fibroblasts, and we have applied high-throughput genomic, proteomic and metabolomic technologies for a systems level analysis. The cellular characterizations reported here provide the background for application of a systems analysis to integrate transcription, post-translational modifications and metabolic activity induced by exposure of cells to ionizing radiation. We present here a summary of the derivation and characterization of cells comprising this model cell system and review applications of this model to systems analysis of ATM functions.
Authors:
Mira Jung; Olga Timofeeva; Amrita K Cheema; Rency Varghese; Habtom Ressom; Anatoly Dritschilo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  720     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-08     Completed Date:  2012-03-29     Revised Date:  2013-08-20    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Affiliation:
Department of Radiation Medicine, Georgetown University Medical Center, Washington, DC 20057, USA. jungm@georgetown.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle Proteins / genetics*,  physiology
Cell Line
DNA-Binding Proteins / genetics*,  physiology
Fibroblasts / metabolism*
Gene Expression Profiling
Humans
Metabolomics
Protein-Serine-Threonine Kinases / genetics*,  physiology
Proteomics
Tumor Suppressor Proteins / genetics*,  physiology
Grant Support
ID/Acronym/Agency:
P01 CA074175/CA/NCI NIH HHS; P01CA74175/CA/NCI NIH HHS; P30CA51008/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Tumor Suppressor Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/ataxia telangiectasia mutated protein
Comments/Corrections

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