Document Detail


Human fetal keratocytes have multipotent characteristics in the developing avian embryo.
MedLine Citation:
PMID:  23461574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; however, there have been no studies demonstrating that human corneal keratocytes are multipotent. The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. HFKs were injected bilaterally into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 and 3, respectively. The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. HuNu-positive keratocytes injected along the neural crest pathway were localized adjacent to HNK-1-positive migratory host neural crest cells and in the cardiac cushion mesenchyme. The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and corneal endothelium. However, they failed to form neurons despite their presence in the condensing trigeminal ganglion. These results show that HFKs retain the ability to differentiate into some neural crest-derived tissues. Their ability to respond to embryonic cues and generate corneal endothelium and stromal keratocytes provides a basis for understanding the feasibility of creating specialized cells for possible use in regenerative medicine.
Authors:
Jennifer R Chao; Marianne E Bronner; Peter Y Lwigale
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-04-05
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-18     Completed Date:  2014-02-05     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2186-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation*
Cell Movement
Cells, Cultured
Chick Embryo
Cornea / cytology,  embryology
Corneal Keratocytes / physiology*,  transplantation
Coronary Vessels / cytology
Endothelium, Corneal / cytology*,  embryology
Fetus / cytology
Humans
Microinjections
Neural Crest / cytology
Skull / blood supply
Trigeminal Ganglion / cytology
Grant Support
ID/Acronym/Agency:
DE16459/DE/NIDCR NIH HHS; EY022158/EY/NEI NIH HHS; K08 EY019714/EY/NEI NIH HHS; K08EY019714/EY/NEI NIH HHS

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