Document Detail

Human eccrine sweat gland cells can reconstitute a stratified epidermis.
MedLine Citation:
PMID:  20376062     Owner:  NLM     Status:  MEDLINE    
Eccrine sweat glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine sweat gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine sweat gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10-12 cell layers is formed by sweat gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the sweat gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the sweat gland-derived epidermis. These data suggest that human eccrine sweat glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine sweat gland cells.
Thomas Biedermann; Luca Pontiggia; Sophie Böttcher-Haberzeth; Sasha Tharakan; Erik Braziulis; Clemens Schiestl; Martin Meuli; Ernst Reichmann
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-08
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  130     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2010-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1996-2009     Citation Subset:  IM    
Tissue Biology Research Unit, Department of Surgery, University Children's Hospital, Zurich, Switzerland.
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MeSH Terms
Biological Markers / metabolism
Cells, Cultured
Child, Preschool
Desmosomes / physiology
Epidermis / cytology*,  physiology,  ultrastructure
Fibroblasts / cytology,  metabolism,  ultrastructure
Homeostasis / physiology
Keratinocytes / cytology,  metabolism,  ultrastructure
Microscopy, Electron, Transmission
Organ Culture Techniques
Skin Transplantation*
Stem Cells / cytology*,  metabolism,  ultrastructure
Sweat Glands / cytology*,  physiology,  ultrastructure
Swiss 3T3 Cells / cytology
Tissue Engineering / methods*
Transplantation, Heterologous*
Reg. No./Substance:
0/Biological Markers

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