Document Detail

Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.
MedLine Citation:
PMID:  14739464     Owner:  NLM     Status:  MEDLINE    
Arabidopsis thaliana De-etiolated-1 (AtDET1) is a highly conserved protein, with orthologs in vertebrate and invertebrate organisms. AtDET1 negatively regulates photomorphogenesis, but its biochemical mechanism and function in other species are unknown. We report that human DET1 (hDET1) promotes ubiquitination and degradation of the proto-oncogenic transcription factor c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1. Ablation of any subunit by RNA interference stabilized c-Jun and increased c-Jun-activated transcription. These findings characterize a c-Jun ubiquitin ligase and define a specific function for hDET1 in mammalian cells.
Ingrid E Wertz; Karen M O'Rourke; Zemin Zhang; David Dornan; David Arnott; Raymond J Deshaies; Vishva M Dixit
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2004-01-22
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  303     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-27     Completed Date:  2004-03-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1371-4     Citation Subset:  IM    
Department of Molecular Oncology, Genentech, Inc., South San Francisco, CA 94080, USA.
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MeSH Terms
Amino Acid Motifs
Amino Acid Sequence
Carrier Proteins / chemistry,  genetics,  metabolism*
Cell Line
Cloning, Molecular
Cullin Proteins / genetics,  metabolism*
DNA-Binding Proteins / metabolism
Genes, jun
Molecular Sequence Data
Nuclear Proteins / chemistry,  genetics,  metabolism
Protein Binding
Proto-Oncogene Proteins c-jun / metabolism*
RNA, Messenger / genetics,  metabolism
RNA, Small Interfering / metabolism
Ubiquitin / metabolism
Ubiquitin-Protein Ligases / chemistry,  metabolism*
Grant Support
Reg. No./Substance:
0/CUL4A protein, human; 0/Carrier Proteins; 0/Cullin Proteins; 0/DDB1 protein, human; 0/DET1 protein, human; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Proto-Oncogene Proteins c-jun; 0/RBX1 protein, human; 0/RFWD2 protein, human; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/Ubiquitin; EC Ligases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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