Document Detail

Human DEAD-box/RNA unwindase rck/p54 contributes to maintenance of cell growth by affecting cell cycle in cultured cells.
MedLine Citation:
PMID:  16773183     Owner:  NLM     Status:  MEDLINE    
Understanding the control of gene expression in cancer cells requires defining the molecular and cellular basis of RNA metabolism compared with that in steady-state normal cells. Previously, we reported evidence that human RNA structure-modifying unwindase rck/p54, a member of the DEAD-box family, was highly expressed in most of the malignant cell lines tested and that this expression was linked to malignant transformation. Here, we show that rck/p54 positively affects cell growth, probably by modulating the gene expression at the translational level in cultured cells. In cell growth and differentiation induced by external stimuli, the level of rck/p54 expression was up-regulated during cell proliferation and down-regulated during differentiation. The down-regulation of rck/p54 in HeLa cells by RNAi induced cell growth inhibition through cell cycle arrest at S phase. Immunoprecipitation using anti-rck/p54 antibody in HeLa cells demonstrated the co-precipitation of rck/p54 with eIF4E, which is well-known to bind to the 5'cap-structure, resulting in initiation of translation. These data suggest that rck/p54 contributes to cell growth possibly by modulating translation-initiation control of the genes involved in the cell proliferation, which is a newly defined mechanism leading to carcinogenesis.
Yukihiro Akao; Kenji Matsumoto; Kenji Ohguchi; Yoshihito Nakagawa; Hitoshi Yoshida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  29     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-14     Completed Date:  2007-08-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  41-8     Citation Subset:  IM    
Gifu International Institute of Biotechnology, Kakamigahara, Gifu 504-0838, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Cycle
Cell Differentiation
Cell Proliferation*
Cell Transformation, Neoplastic / metabolism,  pathology
DEAD-box RNA Helicases / genetics,  metabolism*
Eukaryotic Initiation Factor-4E / metabolism
Hela Cells
Lymphocytes / pathology
Molecular Sequence Data
Promoter Regions, Genetic
Proto-Oncogene Proteins / genetics,  metabolism*
RNA Interference
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism
Transcription Initiation Site
Transcription, Genetic
U937 Cells
Reg. No./Substance:
0/Eukaryotic Initiation Factor-4E; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/RNA, Small Interfering; EC 3.6.1.-/DDX6 protein, human; EC 3.6.1.-/DEAD-box RNA Helicases

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