Document Detail


Human cytomegalovirus (CMV)-induced memory-like NKG2C(+) NK cells are transplantable and expand in vivo in response to recipient CMV antigen.
MedLine Citation:
PMID:  23077239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that NKG2C(+) NK cells from CMV naive umbilical cord blood grafts expand preferentially in recipients after CMV reactivation, representing a primary NK cell response after hematopoietic cell transplantation. In this study, recipients of adult donor hematopoietic cell transplantation were assessed to evaluate the role of donor/recipient CMV serostatus on the expression and function of NKG2C(+) NK cells to determine responses to secondary CMV events. Expansion of NKG2C(+) NK cells was seen following clinical CMV reactivation. However, they also expanded in the absence of detectable CMV viremia when both the donor and recipient were CMV seropositive. Upregulation of NKG2C was observed in NK cells from CMV-positive recipients receiving grafts from CMV-seropositive or -seronegative donors. These in vivo-expanded NKG2C(+) NK cells had an increased capacity for target cell-induced cytokine production, expressed an inhibitory killer Ig-like receptor for self-HLA and preferentially acquired CD57. Most importantly, NKG2C(+) NK cells transplanted from seropositive donors exhibit heightened function in response to a secondary CMV event compared with NKG2C(+) NK cells from seronegative donors. We conclude that NKG2C(+) memory-like NK cells are transplantable and require active or latent (subclinical) expression of CMV Ag in the recipient for clonal expansion of NK cells previously exposed to CMV in the donor.
Authors:
Bree Foley; Sarah Cooley; Michael R Verneris; Julie Curtsinger; Xianghua Luo; Edmund K Waller; Claudio Anasetti; Daniel Weisdorf; Jeffrey S Miller
Related Documents :
23625189 - Tbx3 and nr5α2 play important roles in pig pluripotent stem cells.
23044029 - Therapeutic potential of human induced pluripotent stem cells in experimental stroke.
23245119 - 285 generation of canine induced pluripotent stem cells from canine fetal fibroblast an...
23293299 - Functional evaluation of es cell-derived endodermal populations reveals differences bet...
24315439 - Functional repair of cftr by crispr/cas9 in intestinal stem cell organoids of cystic fi...
23804709 - Myeloid-derived suppressor cells play crucial roles in the regulation of mouse collagen...
12482839 - Mast cell tryptase degrades hdl and blocks its function as an acceptor of cellular chol...
9893019 - In vitro expression of soluble and cell surface-associated cd44 on endometrial cells fr...
3100459 - Generation of activated killer cells in tumor-bearing hosts.
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-17
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  189     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-05     Completed Date:  2013-01-15     Revised Date:  2014-06-10    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5082-8     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD57 / immunology
Antigens, Viral / immunology*
Cells, Cultured
Cytomegalovirus / immunology*,  metabolism
Cytomegalovirus Infections / immunology*,  therapy
Female
Hematologic Neoplasms / immunology,  therapy*
Humans
Immunologic Memory*
Killer Cells, Natural / immunology*,  metabolism,  transplantation
Male
NK Cell Lectin-Like Receptor Subfamily C*
Peripheral Blood Stem Cell Transplantation*
Transplantation, Homologous
Up-Regulation / immunology
Grant Support
ID/Acronym/Agency:
P01 CA065493/CA/NCI NIH HHS; P01 CA111412/CA/NCI NIH HHS; P01-CA111412/CA/NCI NIH HHS; P01-CA65493/CA/NCI NIH HHS; P30 CA077598/CA/NCI NIH HHS; P30-CA77598/CA/NCI NIH HHS; U10 HL069294/HL/NHLBI NIH HHS; U10HL069294/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD57; 0/Antigens, Viral; 0/KLRC2 protein, human; 0/NK Cell Lectin-Like Receptor Subfamily C
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protein kinase C-? clustering at immunological synapses amplifies effector responses in NK cells.
Next Document:  Stimulation of Human EBV- and CMV-Specific Cytolytic Effector Function Using Allogeneic HLA Molecule...