| Human Cdc45 is a proliferation-associated antigen. | |
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MedLine Citation:
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PMID: 17608804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell division cycle protein 45 (Cdc45) plays a critical role in DNA replication to ensure that chromosomal DNA is replicated only once per cell cycle. We analysed the expression of human Cdc45 in proliferating and nonproliferating cells. Our findings show that Cdc45 protein is absent from long-term quiescent, terminally differentiated and senescent human cells, although it is present throughout the cell cycle of proliferating cells. Moreover, Cdc45 is much less abundant than the minichromosome maintenance (Mcm) proteins in human cells, supporting the concept that origin binding of Cdc45 is rate limiting for replication initiation. We also show that the Cdc45 protein level is consistently higher in human cancer-derived cells compared with primary human cells. Consequently, tumour tissue is preferentially stained using Cdc45-specific antibodies. Thus, Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology. |
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Authors:
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S Pollok; C Bauerschmidt; J Sänger; H-P Nasheuer; F Grosse |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-03 |
Journal Detail:
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Title: The FEBS journal Volume: 274 ISSN: 1742-464X ISO Abbreviation: FEBS J. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-13 Completed Date: 2007-08-30 Revised Date: 2011-10-03 |
Medline Journal Info:
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Nlm Unique ID: 101229646 Medline TA: FEBS J Country: England |
Other Details:
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Languages: eng Pagination: 3669-84 Citation Subset: IM |
Affiliation:
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Leibniz Institute for Age Research, Fritz Lipmann Institute, Jena, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens
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genetics,
immunology*,
metabolism* Cell Aging / physiology Cell Cycle Proteins / genetics, immunology*, metabolism* Cell Differentiation Cell Line, Tumor Cell Proliferation Gene Expression Regulation Humans Immunohistochemistry Ki-67 Antigen / metabolism Proliferating Cell Nuclear Antigen / metabolism Tumor Markers, Biological |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/CDC45 protein, human; 0/Cell Cycle Proteins; 0/Ki-67 Antigen; 0/Proliferating Cell Nuclear Antigen; 0/Tumor Markers, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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