| Human Asf1 regulates the flow of S phase histones during replicational stress. | |
| | |
MedLine Citation:
|
PMID: 15664198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Maintenance of chromosomal integrity requires tight coordination of histone biosynthesis with DNA replication. Here, we show that extracts from human cells exposed to replication stress display an increased capacity to support replication-coupled chromatin assembly. While in unperturbed S phase, hAsf1 existed in equilibrium between an active form and an inactive histone-free pool, replication stress mobilized the majority of hAsf1 into an active multichaperone complex together with histones. This active multichaperone complex was limiting for chromatin assembly in S phase extracts, and hAsf1 was required for the enhanced assembly activity in cells exposed to replication stress. Consistently, siRNA-mediated knockdown of hAsf1 impaired the kinetics of S phase progression. Together, these data suggest that hAsf1 provides the cells with a buffering system for histone excess generated in response to stalled replication and explains how mammalian cells maintain a critical "active" histone pool available for deposition during recovery from replication stresses. |
| | |
Authors:
|
Anja Groth; Dominique Ray-Gallet; Jean-Pierre Quivy; Jiri Lukas; Jiri Bartek; Geneviève Almouzni |
Related Documents
:
|
20828558 - Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cel... 8993038 - Ligation-mediated pcr for chromatin-structure analysis of interphase and metaphase chro... 19609198 - The novel histone deacetylase inhibitors metacept-1 and metacept-3 potently increase hi... 7083048 - Differences in protein content of sister nuclei: evidence from binucleate and mononucle... 10648418 - Hodgkin and reed-sternberg-like cells in b-cell chronic lymphocytic leukemia represent ... 1108728 - Sythesis and turnover of phospholipids in escherichia coli cells with inhibited dna syn... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular cell Volume: 17 ISSN: 1097-2765 ISO Abbreviation: Mol. Cell Publication Date: 2005 Jan |
Date Detail:
|
Created Date: 2005-01-24 Completed Date: 2005-03-18 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9802571 Medline TA: Mol Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 301-11 Citation Subset: IM |
Affiliation:
|
Institute of Cancer Biology, The Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Cycle Proteins / genetics, metabolism* Cell Fractionation Cell Line, Tumor Chromatin / metabolism DNA Replication* Histones / metabolism* Humans Hydroxyurea / metabolism Macromolecular Substances Molecular Chaperones / genetics, metabolism Nucleic Acid Synthesis Inhibitors / metabolism S Phase / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/ASF1A protein, human; 0/Cell Cycle Proteins; 0/Chromatin; 0/Histones; 0/Macromolecular Substances; 0/Molecular Chaperones; 0/Nucleic Acid Synthesis Inhibitors; 127-07-1/Hydroxyurea |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: LexA cleavage is required for CTX prophage induction.
Next Document: An important role for RNase R in mRNA decay.