Document Detail


Human aqueous humor phosphatase activity in cataract and glaucoma.
MedLine Citation:
PMID:  22323459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate the presence and activity of protein phosphatase-2A (PPase2A), protein phosphatase-2C (PPase2C), and protein tyrosine phosphatases (PTPs) in the human aqueous humor (AH) of patients with primary open-angle glaucoma (POAG) and cataract and to study the correlation between these phosphatases and the redox state of the AH.
METHODS: Eighty-six cataract patients and 29 POAG patients who were scheduled for cataract surgery with or without glaucoma surgery were enrolled in the study. PPase2A, PPase2C, and PTPs levels in AH were measured by enzyme-linked immunosorbent assays, Western blot analyses, and spectral
METHODS: The redox state was measured by spectral and fluorescent methods.
RESULTS: Phosphatase activity-positive results were significantly higher in AH samples from the POAG group (PP2A χ(2)(1) = 11.754, P < 0.01; PP2C χ(2)(1) = 8.754, P < 0.01; PTP χ(2)(1) = 11.073, P < 0.01). Western blot analysis revealed higher PP2C levels in the AH of glaucoma patients compared with PP2C levels in the AH of cataract patients (P = 0.012). Both oxidized/reduced glutathione ratios and superoxide dismutase levels in the AH were significantly higher in the glaucoma group than in the cataract group. Finally significant correlations were found between PP2A and PP2C, PP2A and PTP, and total antioxidant activity and PTP levels.
CONCLUSIONS: There is a statistically significant difference between phosphatase levels in the AH of POAG patients and cataract patients. The phosphatase content of the AH represents tissue pathology, but their presence in the AH may be attributed to cell debris or to active signaling to other molecular events.
Authors:
Galina Latarya; Ahmad Mansour; Irena Epstein; Daniel Cotlear; Joseph Pikkel; Shmuel Levartovsky; Michael Yulish; Elie Beit-Yannai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-26
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-05-09     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1679-84     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, The Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Aqueous Humor / chemistry,  enzymology*
Blotting, Western
Cataract / metabolism*
Enzyme-Linked Immunosorbent Assay
Female
Glaucoma, Open-Angle / metabolism*
Glutathione / analysis
Humans
Male
Middle Aged
Oxidation-Reduction
Phosphoprotein Phosphatases / analysis,  metabolism
Protein Phosphatase 2 / analysis,  metabolism*
Protein Tyrosine Phosphatases / analysis,  metabolism*
Superoxide Dismutase / analysis
Chemical
Reg. No./Substance:
70-18-8/Glutathione; EC 1.15.1.1/Superoxide Dismutase; EC 3.1.3.16/Phosphoprotein Phosphatases; EC 3.1.3.16/Protein Phosphatase 2; EC 3.1.3.16/protein phosphatase 2C; EC 3.1.3.48/Protein Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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