| Hsp70B' regulation and function. | |
| | |
MedLine Citation:
|
PMID: 18229458 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Heat shock protein (Hsp) 70B' is a human Hsp70 chaperone that is strictly inducible, having little or no basal expression levels in most cells. Using siRNAs to knockdown Hsp70B' and Hsp72 in HT-29, SW-480, and CRL-1807 human colon cell lines, we have found that the two are regulated coordinately in response to stress. We also have found that proteasome inhibition is a potent activator of hsp70B'. Flow cytometry was used to assay hsp70B' promoter activity in HT-29eGFP cells in this study. Knockdown of both Hsp70B' and Hsp72 sensitized cells to heat stress and increasing concentrations of proteasome inhibitor. These data support the conclusion that Hsp72 is the primary Hsp70 family responder to increasing levels of proteotoxic stress, and Hsp70B' is a secondary responder. Interestingly ZnSO4 induces Hsp70B' and not Hsp72 in CRL-1807 cells, suggesting a stressor-specific primary role for Hsp70B'. Both Hsp70B' and Hsp72 are important for maintaining viability under conditions that increase the accumulation of damaged proteins in HT-29 cells. These findings are likely to be important in pathological conditions in which Hsp70B' contributes to cell survival. |
| | |
Authors:
|
Emily J Noonan; Robert F Place; Charles Giardina; Lawrence E Hightower |
Related Documents
:
|
8370318 - Further evidence for two modes of hypothermia damage. 16664328 - Photosynthetic and respiratory rates of two psychrophilic diatoms. 8361618 - Heat shock partially protects rat pheochromocytoma pc12 cells from amyloid beta peptide... 6178178 - Loss of hla-c locus specificities from stored lymphocytes and de novo synthesis followi... 16458288 - Control by cholinergic mechanisms. 18997278 - Selenium is critical for cancer-signaling gene expression but not cell proliferation in... |
Publication Detail:
|
Type: Corrected and Republished Article; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell stress & chaperones Volume: 12 ISSN: 1355-8145 ISO Abbreviation: Cell Stress Chaperones Publication Date: 2007 |
Date Detail:
|
Created Date: 2008-01-30 Completed Date: 2008-03-17 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 9610925 Medline TA: Cell Stress Chaperones Country: United States |
Other Details:
|
Languages: eng Pagination: 393-402 Citation Subset: IM |
Affiliation:
|
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269-3125, USA. emily.noonan@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Agents
/
pharmacology Cell Survival / drug effects Flow Cytometry Green Fluorescent Proteins / metabolism HSP70 Heat-Shock Proteins / metabolism* HSP72 Heat-Shock Proteins / metabolism HT29 Cells Heat-Shock Response / drug effects Humans Leupeptins / pharmacology Promoter Regions, Genetic / genetics Proteasome Endopeptidase Complex / antagonists & inhibitors RNA, Small Interfering / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
ES03828/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/HSP70 Heat-Shock Proteins; 0/HSP72 Heat-Shock Proteins; 0/HSPA7 protein, human; 0/Leupeptins; 0/RNA, Small Interfering; 0/enhanced green fluorescent protein; 133407-82-6/benzyloxycarbonylleucyl-leucyl-leucine aldehyde; 147336-22-9/Green Fluorescent Proteins; EC 3.4.25.1/Proteasome Endopeptidase Complex |
| Comments/Corrections | |
Corrected and Republished From:
|
Cell Stress Chaperones. 2007 Autumn;12(3):219-29
[PMID:
17915554
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plasma heat shock protein 60 and cardiovascular disease risk: the role of psychosocial, genetic, and...
Next Document: Novel insights in the treatment of diabetic nephropathy.