Document Detail


Hsp70 expression and metabolite composition in response to short-term thermal changes in Folsomia candida (Collembola).
MedLine Citation:
PMID:  20601056     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
In the present study the joint transcriptomic and metabolomic responses in Folsomia candida (Collembola) to temperature changes on a short-term scale were studied. Change in heat tolerance was examined as survival after a 35 degrees C heat shock (2h) in the course of either a fluctuating temperature regime (8 to 32 degrees C; pre-treated) or a constant temperature (20 degrees C; control) over a period of 24h. Exposure to a temperature increase from 20 to 32 degrees C (2.4 degrees C min(-)(1)) induced a significantly increased heat tolerance which continued throughout the experiment. Expression of the gene encoding heat shock protein Hsp70 was assessed at the mRNA level using real time quantitative polymerase chain reaction (QPCR). Hsp70 was rapidly induced and significantly increased by the temperature increase. The relative concentrations of low molecular weight metabolites were analysed in F. candida using nuclear magnetic resonance spectroscopy ((1)H NMR). A significant metabolomic divergence between pre-treated and control collembolans was evident; partly due to a significantly reduced relative concentration of five free amino acids (arginine, leucine, lysine, phenylalanine and tyrosine) in pre-treated collembolans. These results, obtained from ecological transcriptomics and metabolomics jointly generated insight on various levels into the combined responses to a changing environment.
Authors:
Dorthe Waagner; Lars-Henrik Heckmann; Anders Malmendal; Niels Chr Nielsen; Martin Holmstrup; Mark Bayley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-17
Journal Detail:
Title:  Comparative biochemistry and physiology. Part A, Molecular & integrative physiology     Volume:  157     ISSN:  1531-4332     ISO Abbreviation:  Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-07-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806096     Medline TA:  Comp Biochem Physiol A Mol Integr Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  177-83     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Aarhus University, Department of Biological Sciences, Aarhus C, Denmark.
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