| The Hsp40 molecular chaperone Ydj1p, along with the protein kinase C pathway, affects cell-wall integrity in the yeast Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 17237519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecular chaperones, such as Hsp40, regulate cellular processes by aiding in the folding, localization, and activation of multi-protein machines. To identify new targets of chaperone action, we performed a multi-copy suppressor screen for genes that improved the slow-growth defect of yeast lacking the YDJ1 chromosomal locus and expressing a defective Hsp40 chimera. Among the genes identified were MID2, which regulates cell-wall integrity, and PKC1, which encodes protein kinase C and is linked to cell-wall biogenesis. We found that ydj1delta yeast exhibit phenotypes consistent with cell-wall defects and that these phenotypes were improved by Mid2p or Pkc1p overexpression or by overexpression of activated downstream components in the PKC pathway. Yeast containing a thermosensitive allele in the gene encoding Hsp90 also exhibited cell-wall defects, and Mid2p or Pkc1p overexpression improved the growth of these cells at elevated temperatures. To determine the physiological basis for suppression of the ydj1delta growth defect, wild-type and ydj1delta yeast were examined by electron microscopy and we found that Mid2p overexpression thickened the mutant's cell wall. Together, these data provide the first direct link between cytoplasmic chaperone function and cell-wall integrity and suggest that chaperones orchestrate the complex biogenesis of this structure. |
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Authors:
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Christine M Wright; Sheara W Fewell; Mara L Sullivan; James M Pipas; Simon C Watkins; Jeffrey L Brodsky |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-01-21 |
Journal Detail:
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Title: Genetics Volume: 175 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-23 Completed Date: 2007-06-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 1649-64 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Calcium-Binding Proteins / genetics, metabolism Cell Wall / metabolism, ultrastructure DNA, Fungal / genetics Genes, Fungal HSP40 Heat-Shock Proteins / genetics*, metabolism* HSP90 Heat-Shock Proteins / genetics, metabolism Intracellular Signaling Peptides and Proteins Membrane Glycoproteins Membrane Proteins / genetics, metabolism Mutation Phenotype Plasmids / genetics Protein Kinase C / genetics*, metabolism* Saccharomyces cerevisiae / genetics*, growth & development, metabolism*, ultrastructure Saccharomyces cerevisiae Proteins / genetics*, metabolism* Suppression, Genetic Temperature |
| Grant Support | |
ID/Acronym/Agency:
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DK61296/DK/NIDDK NIH HHS; GM75061/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Proteins; 0/DNA, Fungal; 0/HSP40 Heat-Shock Proteins; 0/HSP82 protein, S cerevisiae; 0/HSP90 Heat-Shock Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/MID2 protein, S cerevisiae; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Saccharomyces cerevisiae Proteins; 139874-78-5/YDJ1 protein, S cerevisiae; EC 2.7.1.37/PKC1 protein, S cerevisiae; EC 2.7.11.13/Protein Kinase C |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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