Document Detail

Hsp27 decreases inclusion body formation from mutated GTP-cyclohydrolase I protein.
MedLine Citation:
PMID:  18241680     Owner:  NLM     Status:  MEDLINE    
GTP cyclohydrolase I (GCH), an oligomeric protein composed of 10 identical subunits, is required for the synthesis of neurotransmitters; mutations in GCH are associated with dopa-responsive dystonia (DRD) and hyperphenylalaninemia. Mutated GCH proteins are unstable and prone to dominant-negative effect. We show herein that expression of the GCH mutant GCH-201E or the splicing variant GCH-II caused intracellular inclusion bodies. When Hsp27 was expressed together with the GCH mutants, Hsp27 expression decreased the formation of inclusion bodies by GCH (as assessed by immunofluorescence) and decreased the amount of insoluble GCH mutant proteins (as assessed by Western blot). Transfection of pcDNA-Hsp27-S3D, a phosphorylation-mimicry Hsp27 mutant, was more effective at the mutated GCH proteins than transfection with pcDNA-Hsp27, but okadaic acid, a phosphatase inhibitor, enhanced the effect of pcDNA-Hsp27. Hsp27-S3D also abolished the dominant-negative action of GCH-II. The mutated GCH proteins interacted with the wild-type GCH protein; the inclusion bodies were positive for lysosomal marker LAMP1, soluble in 2% SDS, and were not ubiquitinated. Phophorlyated Hsp27 also decreased the inclusion body formation by the huntingtin polyglutamines. Therefore, diseases involving mutated oligomeric proteins would be manageable by chaperone therapies.
Yu-Wei Chiou; Wuh-Liang Hwu; Yu-May Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-14
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1782     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-19     Completed Date:  2008-04-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  169-79     Citation Subset:  IM    
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
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MeSH Terms
Cells, Cultured
Fluorescent Antibody Technique
GTP Cyclohydrolase / genetics,  physiology*
HSP27 Heat-Shock Proteins
Heat-Shock Proteins / pharmacology*
Inclusion Bodies / drug effects*
Neoplasm Proteins / pharmacology*
Okadaic Acid / pharmacology
Reg. No./Substance:
0/HSP27 Heat-Shock Proteins; 0/HSPB1 protein, human; 0/Heat-Shock Proteins; 0/Neoplasm Proteins; 78111-17-8/Okadaic Acid; EC Cyclohydrolase

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