Document Detail


Hsp25-induced radioresistance is associated with reduction of death by apoptosis: involvement of Bcl2 and the cell cycle.
MedLine Citation:
PMID:  11023606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously demonstrated the protective effect of the small heat-shock protein against oxidative damage induced by tumor necrosis factor alpha. Here we have extended our studies of the possible role of Hsp25 in ionizing radiation-induced damage. For these studies, we transfected murine fibroblast L929 cells with the Hsp25 gene and selected three stably transfected clones. Hsp25 overexpression conferred radioresistance as detected by clonogenic survival and induction of apoptosis. Interestingly, the Hsp25-transfected cells showed an increase in the level of the anti-apoptosis molecule Bcl2. We also observed alterations of cell growth in the Hsp25-transfected cells. The cell cycle time of Hsp25-transfected cells was 3-4 h slower than that of vector-transfected control cells. Flow cytometry analysis of synchronized cells at late G(1) phase by mimosine treatment also showed the growth delay in Hsp25-overexpressing cells. In addition, reduced cyclin D1, cyclin A and Cdc2 levels and increased levels of Cdkn1a (also known as p21(Waf)) were observed in Hsp25-transfected cells, which probably caused the reduction in cell growth. In addition, synchronization by mimosine treatment only partially altered radioresistance in the Hsp25-transfected cells. Taken together, these data suggest that Hsp25-induced radioresistance is associated with growth delay as well as induction of Bcl2.
Authors:
S H Park; H N Cho; S J Lee; T H Kim; Y Lee; Y M Park; Y J Lee; C K Cho; S Y Yoo; Y S Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  154     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  421-8     Citation Subset:  IM; S    
Affiliation:
Laboratory of Radiation Effect, Korea Cancer Center Hospital, 215-4 Gongeung-Dong, Nowon-Ku, Seoul 139-706, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis* / radiation effects
Cell Cycle* / radiation effects
Cell Cycle Proteins / metabolism
Colony-Forming Units Assay
Gamma Rays
Gene Expression Regulation / radiation effects
Genes, bcl-2
Heat-Shock Proteins*
L Cells (Cell Line) / cytology,  metabolism,  radiation effects
Mice
Mimosine / pharmacology
Neoplasm Proteins / biosynthesis,  genetics,  physiology*
Oxidative Stress
Proto-Oncogene Proteins c-bcl-2 / biosynthesis,  physiology*
Radiation Tolerance / drug effects,  physiology*
Recombinant Fusion Proteins / physiology
Transfection
Grant Support
ID/Acronym/Agency:
CA48000/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Heat-Shock Proteins; 0/Hspb1 protein, mouse; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Recombinant Fusion Proteins; 500-44-7/Mimosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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