Document Detail


Hoxa2 downregulates Six2 in the neural crest-derived mesenchyme.
MedLine Citation:
PMID:  15634706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Hoxa2 transcription factor acts during development of the second branchial arch. As for most of the developmental processes controlled by Hox proteins, the mechanism by which Hoxa2 regulates the morphology of second branchial arch derivatives is unclear. We show that Six2, another transcription factor, is genetically downstream of Hoxa2. High levels of Six2 are observed in the Hoxa2 loss-of-function mutant. By using a transgenic approach to overexpress Six2 in the embryonic area controlled by Hoxa2, we observed a phenotype that is reminiscent of the Hoxa2 mutant phenotype. Furthermore, we demonstrate that Hoxa2 regulation of Six2 is confined to a 0.9 kb fragment of the Six2 promoter and that Hoxa2 binds to this promoter region. These results strongly suggest that Six2 is a direct target of Hoxa2.
Authors:
Eva Kutejova; Bettina Engist; Moisés Mallo; Benoît Kanzler; Nicoletta Bobola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-05
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  132     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-17     Completed Date:  2005-03-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  469-78     Citation Subset:  IM    
Affiliation:
Department of Developmental Biology, Max-Planck Institute of Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Branchial Region / embryology,  metabolism
Down-Regulation*
Gene Expression Regulation, Developmental*
Head / embryology
Homeodomain Proteins / genetics,  metabolism*
Mesoderm / cytology,  metabolism*
Mice
Mice, Transgenic
Molecular Sequence Data
Mutation / genetics
Neural Crest / cytology,  embryology*,  metabolism*
Phenotype
Promoter Regions, Genetic / genetics
RNA, Messenger / genetics,  metabolism
Transcription Factors / chemistry,  genetics,  metabolism*
Up-Regulation / genetics
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Hoxa2 protein, mouse; 0/RNA, Messenger; 0/Six2 protein, mouse; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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