Document Detail

How deeply cells feel: methods for thin gels.
MedLine Citation:
PMID:  20454525     Owner:  NLM     Status:  MEDLINE    
Tissue cells lack the ability to see or hear but have evolved mechanisms to feel into their surroundings and sense a collective stiffness. A cell can even sense the effective stiffness of rigid objects that are not in direct cellular contact - like the proverbial princess who feels a pea placed beneath soft mattresses. How deeply a cell feels into a matrix can be measured by assessing cell responses on a controlled series of thin and elastic gels that are affixed to a rigid substrate. Gel elasticity E is readily varied with polymer concentrations of now-standard polyacrylamide hydrogels, but to eliminate wrinkling and detachment of thin gels from an underlying glass coverslip, vinyl groups are bonded to the glass before polymerization. Gel thickness is nominally specified using micron-scale beads that act as spacers, but gels swell after polymerization as measured by z-section, confocal microscopy of fluorescent gels. Atomic force microscopy (AFM) is used to measure E at gel surfaces, employing stresses and strains that are typically generated by cells and yielding values for E that span a broad range of tissue microenvironments. To illustrate cell sensitivities to a series of thin-to-thick gels, the adhesive spreading of mesenchymal stem cells was measured on gel mimics of a very soft tissue (eg. brain, E ~ 1 kPa). Initial results show that cells increasingly respond to the rigidity of an underlying 'hidden' surface starting at about 10-20 microm gel thickness with a characteristic tactile length of less than about 5 microm.
Amnon Buxboim; Karthikan Rajagopal; Andre' E X Brown; Dennis E Discher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of physics. Condensed matter : an Institute of Physics journal     Volume:  22     ISSN:  1361-648X     ISO Abbreviation:  J Phys Condens Matter     Publication Date:  2010 May 
Date Detail:
Created Date:  2011-03-09     Completed Date:  2011-08-10     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  101165248     Medline TA:  J Phys Condens Matter     Country:  England    
Other Details:
Languages:  eng     Pagination:  194116     Citation Subset:  IM    
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MeSH Terms
Acrylic Resins / chemistry*
Biomimetic Materials / chemistry*
Cell Adhesion / physiology*
Elastic Modulus
Focal Adhesions / physiology*
Mechanotransduction, Cellular / physiology*
Mesenchymal Stromal Cells / physiology*
Shear Strength / physiology*
Stress, Mechanical
Surface Properties
Grant Support
P01 DK032094/DK/NIDDK NIH HHS; P01 DK032094-20/DK/NIDDK NIH HHS; P01 DK032094-21/DK/NIDDK NIH HHS; P01 DK032094-22/DK/NIDDK NIH HHS; P01 DK032094-23/DK/NIDDK NIH HHS; P01 DK032094-24A1/DK/NIDDK NIH HHS; P01 DK032094-24A15589/DK/NIDDK NIH HHS; P01 DK032094-25/DK/NIDDK NIH HHS; R01 HL062352/HL/NHLBI NIH HHS; R01 HL062352-09A1/HL/NHLBI NIH HHS; R01 HL062352-10/HL/NHLBI NIH HHS; R21 AR056128/AR/NIAMS NIH HHS; R21 AR056128-01A1/AR/NIAMS NIH HHS; R21 AR056128-02/AR/NIAMS NIH HHS; R21 EB004489/EB/NIBIB NIH HHS; R21 EB004489-01/EB/NIBIB NIH HHS; R21 EB004489-02/EB/NIBIB NIH HHS; S10 RR022575/RR/NCRR NIH HHS; S10 RR022575-01A1/RR/NCRR NIH HHS
Reg. No./Substance:
0/Acrylic Resins; 0/polyacrylamide gels

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