| How can biochemical reactions within cells differ from those in test tubes? | |
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MedLine Citation:
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PMID: 16825427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nonspecific interactions between individual macro-molecules and their immediate surroundings ("background interactions") within a medium as heterogeneous and highly volume occupied as the interior of a living cell can greatly influence the equilibria and rates of reactions in which they participate. Background interactions may be either repulsive, leading to preferential size-and-shape-dependent exclusion from highly volume-occupied elements of volume, or attractive, leading to nonspecific associations or adsorption. Nonspecific interactions with different constituents of the cellular interior lead to three classes of phenomena: macromolecular crowding, confinement and adsorption. Theory and experiment have established that predominantly repulsive background interactions tend to enhance the rate and extent of macromolecular associations in solution, whereas predominantly attractive background interactions tend to enhance the tendency of macromolecules to associate on adsorbing surfaces. Greater than order-of-magnitude increases in association rate and equilibrium constants attributable to background interactions have been observed in simulated and actual intracellular environments. |
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Authors:
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Allen P Minton |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Journal of cell science Volume: 119 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-07 Completed Date: 2006-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 2863-9 Citation Subset: IM |
Affiliation:
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Section on Physical Biochemistry, Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health/U.S. DHHS, Bethesda, MD 20892, USA. minton@helix.nih.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell-Free System Cells / cytology*, metabolism* Macromolecular Substances / metabolism Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances |
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