Document Detail


How Giardia swim and divide.
MedLine Citation:
PMID:  11705969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine how binuclear giardia swim, we used video microscopy to observe trophozoites of Giardia intestinalis, which were labeled with an amino-specific Alexa Fluor dye that highlighted the flagella and adherence disc. Giardia swam forward by means of the synchronous beating of anterior, posterolateral, and ventral flagella in the plane of the ventral disc, while caudal flagella swam in a plane perpendicular to the disc. Giardia turned in the plane of the disc by means of a rudder-like motion of its tail, which was constant rather than beating. To determine how giardia divide, we used three-dimensional confocal microscopy, the same surface label, nuclear stains, and antitubulin antibodies. Giardia divided with mirror-image symmetry in the plane of the adherence disc, so that the right nucleus of the mother became the left nucleus of the daughter. Pairs of nuclei were tethered together by microtubules which surrounded nuclei and prevented mother or daughter giardia from receiving two copies of the same nucleus. New adherence discs formed upon a spiral backbone of microtubules, which had a clockwise rotation when viewed from the ventral surface. These dynamic observations of the parasite begin to reveal how giardia swim and divide.
Authors:
S Ghosh; M Frisardi; R Rogers; J Samuelson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  69     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-13     Completed Date:  2001-12-12     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7866-72     Citation Subset:  IM    
Affiliation:
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Division
Cell Movement / physiology*
Cell Nucleus / physiology,  ultrastructure
Cytoskeleton / ultrastructure
Flagella / physiology
Fluorescent Dyes
Giardia lamblia / cytology*,  physiology*,  ultrastructure
Microscopy, Video
Grant Support
ID/Acronym/Agency:
2HL-43510/HL/NHLBI NIH HHS; AI-33492/AI/NIAID NIH HHS; HL-33009/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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