Document Detail


Houttuynia cordata Thunb extract inhibits cell growth and induces apoptosis in human primary colorectal cancer cells.
MedLine Citation:
PMID:  20944136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is reported that Houttuynia cordata Thunb. (HCT), a traditional Chinese herbal medicine, has many biological properties such as antiviral, antibacterial and antileukemic activities. However, the molecular mechanisms of cytotoxicity and apoptosis in human primary colorectal cancer cells are not clear. In this study, whether HCT induced cytotoxicity in primary colorectal cancer cells obtained from three patients was investigated. The results indicated that HCT inhibited growth of cancer cells in a dose-dependent manner. After treatment with HCT (250 μg/ml) for 24 h, cells exhibited chromatin condensation (an apoptotic characteristic). HCT increased reactive oxygen species (ROS) production and decreased the mitochondrial membrane potential (ΔΨ(m)) in examined cells. Mitochondria-dependent apoptotic signaling pathway was shown to be involved as determined by increase in the levels of cytochrome c, Apaf-1, and caspase-3 and -9. The decrease in the level of ΔΨ(m) was associated with an increase in the BAX/BCL-2 ratio which led to activation of caspase-9 and -3. Based on our results, HCT induced apoptotic cell death in human primary colorectal cancer cells through a mitochondria-dependent signaling pathway.
Authors:
Kuang-Chi Lai; Yu-Jen Chiu; Yih-Jing Tang; Kuei-Li Lin; Jo-Hua Chiang; Yi-Lin Jiang; Hsiu-Fang Jen; Yueh-Hsiung Kuo; Sakae Agamaya; Jing-Gung Chung; Jai-Sing Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3549-56     Citation Subset:  IM    
Affiliation:
China Medical University, No 91, Hsueh-Shih Road, Taichung 404, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Blotting, Western
Caspases / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
Cell Survival / drug effects
Colorectal Neoplasms / metabolism,  pathology*
Drugs, Chinese Herbal / pharmacology*
Humans
Membrane Potential, Mitochondrial / drug effects
Reactive Oxygen Species / metabolism
Signal Transduction / drug effects
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Drugs, Chinese Herbal; 0/Reactive Oxygen Species; 0/plant extract, Houttuynia cordata; EC 3.4.22.-/Caspases

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