Document Detail


Houseflies: not simple mechanical vectors of enterohemorrhagic Escherichia coli O157:H7.
MedLine Citation:
PMID:  10548298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An epidemic of enterohemorrhagic colitis caused by Escherichia coli O157:H7 (EHEC-O157) occurred in a nursery school in a rural area of Japan in September 1996. The EHEC-O157 were isolated both from patients and houseflies collected at the school. The flies were suspected to be mechanical vectors of the pathogen. Feeding experiments of EHEC-O157 to houseflies showed that the ingested bacteria were harbored in the intestine of flies and continued to be excreted at least for 3 days after feeding. Scanning electron microscopy showed that a large number of EHEC-O157 adhered to the surface of the housefly mouthparts and actively proliferated in the minute spaces of the labellum. Food masses containing EHEC-O157 in the fly intestine were completely surrounded by a peritrophic membrane during digestion and discharged rapidly. The persistence of bacteria in the intestine and feces is mainly a result of proliferation in the mouthparts and accumulation in the crop. Our results strongly suggest that houseflies are not simple mechanical vectors of EHEC. The epidemiologic potential of houseflies to disseminate EHEC-O157 may be greater than initially suspected.
Authors:
M Kobayashi; T Sasaki; N Saito; K Tamura; K Suzuki; H Watanabe; N Agui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  61     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-16     Completed Date:  1999-11-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  625-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Colony Count, Microbial
Enterocolitis / microbiology
Escherichia coli Infections / transmission*
Escherichia coli O157 / pathogenicity*,  ultrastructure
Feces / microbiology
Female
Gastrointestinal Hemorrhage / microbiology
Houseflies / microbiology*,  physiology,  ultrastructure
Humans
Insect Vectors / microbiology*,  physiology,  ultrastructure
Intestines / microbiology
Microscopy, Electron
Microscopy, Electron, Scanning
Microscopy, Immunoelectron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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