| House dust induces IL-6 and IL-8 response in A549 epithelial cells. | |
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MedLine Citation:
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PMID: 10649856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The in vitro potency of house dust to induce cytokine response in A549 lung epithelial cells was studied. Dusts collected from carpet, bed, shelf and floor of a villa and an apartment by vacuuming were found to trigger the production of interleukin-8 (IL-8) and interleukin-6 (IL-6) in a dose-dependent manner, and the interleukin production was several-fold higher than of swine dust (used as a positive control). The IL-8 and IL-6 production of pure Escherichia coli lipopolysaccharide was significantly lower than of the dusts and a peptidoglycan-polysaccharide complex did not show any stimulatory effect at all. The lipopolysaccharide and peptidoglycan contents of the samples were determined by gas chromatography-tandem mass spectrometry analysis of, respectively, 3-hydroxy fatty acids and muramic acid; in addition, ergosterol was monitored for fungal biomass. The inflammatory properties of house dust upon inhalation may be reflected in its high potency to induce cytokine response in lung epithelial cells. |
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Authors:
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A Saraf; L Larsson; B M Larsson; K Larsson; L Palmberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Indoor air Volume: 9 ISSN: 0905-6947 ISO Abbreviation: Indoor Air Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-09 Completed Date: 2000-02-09 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 9423515 Medline TA: Indoor Air Country: DENMARK |
Other Details:
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Languages: eng Pagination: 219-25 Citation Subset: IM |
Affiliation:
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Department of Infectious Diseases and Medical Microbiology, University of Lund, Sweden. Anita.Saraf@mmb.lu.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Pollution, Indoor
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adverse effects* Cell Line Dust* Epithelial Cells / immunology Humans Inflammation Inhalation Exposure Interleukin-6 / biosynthesis*, immunology Interleukin-8 / biosynthesis*, immunology Lung / drug effects, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Dust; 0/Interleukin-6; 0/Interleukin-8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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