Document Detail


Host-microbe interactions in the neonatal intestine: role of human milk oligosaccharides.
MedLine Citation:
PMID:  22585924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The infant intestinal microbiota is shaped by genetics and environment, including the route of delivery and early dietary intake. Data from germ-free rodents and piglets support a critical role for the microbiota in regulating gastrointestinal and immune development. Human milk oligosaccharides (HMO) both directly and indirectly influence intestinal development by regulating cell proliferation, acting as prebiotics for beneficial bacteria and modulating immune development. We have shown that the gut microbiota, the microbial metatranscriptome, and metabolome differ between porcine milk-fed and formula-fed (FF) piglets. Our goal is to define how early nutrition, specifically HMO, shapes host-microbe interactions in breast-fed (BF) and FF human infants. We an established noninvasive method that uses stool samples containing intact sloughed epithelial cells to quantify intestinal gene expression profiles in human infants. We hypothesized that a systems biology approach, combining i) HMO composition of the mother's milk with the infant's gut gene expression and fecal bacterial composition, ii) gene expression, and iii short-chain fatty acid profiles would identify important mechanistic pathways affecting intestinal development of BF and FF infants in the first few months of life. HMO composition was analyzed by HLPC Chip/time-of-flight MS and 3 HMO clusters were identified using principle component analysis. Initial findings indicated that both host epithelial cell mRNA expression and the microbial phylogenetic profiles provided strong feature sets that distinctly classified the BF and FF infants. Ongoing analyses are designed to integrate the host transcriptome, bacterial phylogenetic profiles, and functional metagenomic data using multivariate statistical analyses.
Authors:
Sharon M Donovan; Mei Wang; Min Li; Iddo Friedberg; Scott L Schwartz; Robert S Chapkin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-05-01
Journal Detail:
Title:  Advances in nutrition (Bethesda, Md.)     Volume:  3     ISSN:  2156-5376     ISO Abbreviation:  Adv Nutr     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-15     Completed Date:  2012-10-01     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  101540874     Medline TA:  Adv Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  450S-5S     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL, USA. sdonovan@illinois.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Breast Feeding
Epithelial Cells / metabolism,  microbiology
Fatty Acids, Volatile / analysis
Feces / microbiology
Gene Expression Profiling
Genes, Bacterial
Humans
Infant
Infant Formula / administration & dosage
Intestines / microbiology*
Metagenome*
Milk / chemistry
Milk, Human / chemistry*
Oligosaccharides / administration & dosage*
Phylogeny
Prebiotics / microbiology
Swine
Transcriptome
Grant Support
ID/Acronym/Agency:
R01 CA129444/CA/NCI NIH HHS; R01 HD061929/HD/NICHD NIH HHS; R25T-CA090301/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Volatile; 0/Oligosaccharides; 0/Prebiotics
Comments/Corrections

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