Document Detail


Host cell entry by apicomplexa parasites requires actin polymerization in the host cell.
MedLine Citation:
PMID:  19286135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apicomplexa are obligate intracellular parasites that actively invade host cells using their membrane-associated, actin-myosin motor. The current view is that host cell invasion by Apicomplexa requires the formation of a parasite-host cell junction, which has been termed the moving junction, but does not require the active participation of host actin. Using Toxoplasma gondii tachyzoites and Plasmodium berghei sporozoites, we show that host actin participates in parasite entry. Parasites induce the formation of a ring-shaped F-actin structure in the host cell at the parasite-cell junction, which remains stable during parasite entry. The Arp2/3 complex, an actin-nucleating factor, is recruited at the ring structure and is important for parasite entry. We propose that Apicomplexa invasion of host cells requires not only the parasite motor but also de novo polymerization of host actin at the entry site for anchoring the junction on which the parasite pulls to penetrate the host cell.
Authors:
Virginie Gonzalez; Audrey Combe; Violaine David; Nicholas A Malmquist; Violaine Delorme; Carole Leroy; Samantha Blazquez; Robert Ménard; Isabelle Tardieux
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell host & microbe     Volume:  5     ISSN:  1934-6069     ISO Abbreviation:  Cell Host Microbe     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101302316     Medline TA:  Cell Host Microbe     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-72     Citation Subset:  IM    
Affiliation:
Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Actin-Related Protein 2-3 Complex / analysis
Actins / metabolism*
Animals
Cell Line
Cytoplasm / chemistry
Host-Parasite Interactions*
Humans
Plasmodium berghei / physiology*
Protein Multimerization*
Toxoplasma / physiology*
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Actin-Related Protein 2-3 Complex; 0/Actins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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