| Host blood meal identification by multiplex polymerase chain reaction for dispersal evidence of stable flies (Diptera:Muscidae) between livestock facilities. | |
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MedLine Citation:
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PMID: 21337948 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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A species-specific multiplex polymerase chain reaction targeting the cytochrome b gene of cattle, horses, humans, and dogs was developed to determine the blood meal sources of stable flies, Stomoxys calcitrans (L.), collected from Florida equine facilities. Of 595 presumptive blood-fed stable flies analyzed, successful host amplification was obtained in 350, for a field host-detection efficiency of 58.8%. The majority of analyzed stable flies had fed on cattle (64.6%), followed by horses (24.3%), humans (9.5%), and dogs (1.6%). A survey of animal-enclosed pastures occurring within 3 km of stable fly collection sites revealed that the nearest cattle were between 0.8 and 1.5 km from the four horse farm sampling sites. Cattle-feeding frequencies were greater on farms where cattle were located at distances of 0.8 km, suggesting that between farm differences in host-feeding frequency is related to the number of and distance from a particular host type. Time course evaluations of previously laboratory-fed stable flies demonstrated that host-detection efficiency with this system was 100, 50, and 0% when flies were evaluated at 16, 24, and 48 h postblood feeding, respectively. The results of this study suggest short-term stable fly dispersal of up to 1.5 km in a 48-h time period. The implications of these findings are discussed. |
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Authors:
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Jimmy B Pitzer; Phillip E Kaufman; Saundra H Tenbroeck; James E Maruniak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of medical entomology Volume: 48 ISSN: 0022-2585 ISO Abbreviation: J. Med. Entomol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-02-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375400 Medline TA: J Med Entomol Country: United States |
Other Details:
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Languages: eng Pagination: 53-60 Citation Subset: IM |
Affiliation:
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Entomology and Nematology Department, University of Florida, 970 Natural Area Drive, Gainesville, FL 32611, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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