| Host Proteasomal Degradation Generates Amino Acids Essential for Intracellular Bacterial Growth. | |
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MedLine Citation:
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PMID: 22096100 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Legionella pneumophila proliferates in environmental amoeba and human cells within the Legionella-containing vacuole (LCV). The exported AnkB F-box effector of L. pneumophila is anchored into the LCV membrane by host-mediated farnesylation. Here, we report that host proteasomal degradation of Lys(48)-linked polyubiquitinated proteins, assembled on the LCV by AnkB, generates amino acids required for intracellular bacterial proliferation. The severe defect of the ankB null mutant in proliferation within amoeba and human cells is rescued by supplementation of a mixture of amino acids, or cysteine, serine, pyruvate, or citrate, similar to rescue by genetic complementation. Defect of the ankB mutant in intrapulmonary proliferation in mice is rescued upon injection of a mixture of amino acids or cysteine. Therefore, Legionella promotes eukaryotic proteasomal degradation to generate amino acids needed as carbon and energy sources for bacterial proliferation within evolutionarily distant hosts. |
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Authors:
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Christopher T D Price; Tasneem Al-Quadan; Marina Santic; Ilan Rosenshine; Yousef Abu Kwaik |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-17 |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: - ISSN: 1095-9203 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Microbiology and Immunology, College of Medicine, University of Louisville, Louisville, KY 40202, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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