| Host cell lipid bodies triggered by Trypanosoma cruzi infection and enhanced by the uptake of apoptotic cells are associated with prostaglandin E₂ generation and increased parasite growth. | |
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MedLine Citation:
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PMID: 21849292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipid bodies (lipid droplets) are lipid-rich organelles with functions in cell metabolism and signaling. Here, we investigate the mechanisms of Trypanosoma cruzi-induced lipid body formation and their contributions to host-parasite interplay. We demonstrate that T. cruzi-induced lipid body formation in macrophages occurs in a Toll-like receptor 2-dependent mechanism and is potentiated by apoptotic cell uptake. Lipid body biogenesis and prostaglandin E₂ (PGE₂) production triggered by apoptotic cell uptake was largely dependent of α(v)β₃ and transforming growth factor-β signaling. T. cruzi-induced lipid bodies act as sites of increased PGE synthesis. Inhibition of lipid body biogenesis by the fatty acid synthase inhibitor C75 reversed the effects of apoptotic cells on lipid body formation, eicosanoid synthesis, and parasite replication. Our findings indicate that lipid bodies are highly regulated organelles during T. cruzi infection with roles in lipid mediator generation by macrophages and are potentially involved in T. cruzi-triggered escape mechanisms. |
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Authors:
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Heloisa D'Avila; Célio G Freire-de-Lima; Natalia R Roque; Livia Teixeira; Christina Barja-Fidalgo; Adriana R Silva; Rossana C N Melo; George A Dosreis; Hugo C Castro-Faria-Neto; Patrícia T Bozza |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 204 ISSN: 1537-6613 ISO Abbreviation: J. Infect. Dis. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-18 Completed Date: 2011-10-13 Revised Date: 2011-10-31 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 951-61 Citation Subset: AIM; IM |
Affiliation:
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Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chagas Disease / pathology* Dinoprostone / metabolism* Female Host-Parasite Interactions* Lipid Metabolism* Macrophages / metabolism*, parasitology* Mice Mice, Inbred C57BL Rats Rats, Sprague-Dawley Toll-Like Receptor 2 / metabolism Trypanosoma cruzi / growth & development, pathogenicity* |
| Chemical | |
Reg. No./Substance:
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0/Toll-Like Receptor 2; 363-24-6/Dinoprostone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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