Document Detail


Host cell invasion and virulence mediated by Candida albicans Ssa1.
MedLine Citation:
PMID:  21085601     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Candida albicans Ssa1 and Ssa2 are members of the HSP70 family of heat shock proteins that are expressed on the cell surface and function as receptors for antimicrobial peptides such as histatins. We investigated the role of Ssa1 and Ssa2 in mediating pathogenic host cell interactions and virulence. A C. albicans ssa1Δ/Δ mutant had attenuated virulence in murine models of disseminated and oropharyngeal candidiasis, whereas an ssa2Δ/Δ mutant did not. In vitro studies revealed that the ssa1Δ/Δ mutant caused markedly less damage to endothelial cells and oral epithelial cell lines. Also, the ssa1Δ/Δ mutant had defective binding to endothelial cell N-cadherin and epithelial cell E-cadherin, receptors that mediate host cell endocytosis of C. albicans. As a result, this mutant had impaired capacity to induce its own endocytosis by endothelial cells and oral epithelial cells. Latex beads coated with recombinant Ssa1 were avidly endocytosed by both endothelial cells and oral epithelial cells, demonstrating that Ssa1 is sufficient to induce host cell endocytosis. These results indicate that Ssa1 is a novel invasin that binds to host cell cadherins, induces host cell endocytosis, and is critical for C. albicans to cause maximal damage to host cells and induce disseminated and oropharyngeal disease.
Authors:
Jianing N Sun; Norma V Solis; Quynh T Phan; Jashanjot S Bajwa; Helena Kashleva; Angela Thompson; Yaoping Liu; Anna Dongari-Bagtzoglou; Mira Edgerton; Scott G Filler
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-11-11
Journal Detail:
Title:  PLoS pathogens     Volume:  6     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2010  
Date Detail:
Created Date:  2010-11-18     Completed Date:  2011-03-16     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1001181     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadherins / metabolism
Candida albicans / physiology*
Candidiasis / metabolism,  microbiology*,  pathology*
DNA, Bacterial / genetics
Endocytosis / physiology
Endothelial Cells / metabolism
Epithelial Cells / metabolism
HSP70 Heat-Shock Proteins / genetics,  metabolism*
Male
Mice
Mice, Inbred BALB C / microbiology*
Polymerase Chain Reaction
Virulence / genetics*
Grant Support
ID/Acronym/Agency:
M01RR00425/RR/NCRR NIH HHS; R01 AI054928/AI/NIAID NIH HHS; R01 DE013986/DE/NIDCR NIH HHS; R01 DE013986-09/DE/NIDCR NIH HHS; R01 DE017088/DE/NIDCR NIH HHS; R01AI054928/AI/NIAID NIH HHS; R01DE010641/DE/NIDCR NIH HHS; R01DE017088/DE/NIDCR NIH HHS; R01DE13986/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Cadherins; 0/DNA, Bacterial; 0/HSP70 Heat-Shock Proteins
Comments/Corrections

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