Document Detail


Hormone-sensitive lipase expression and IHC localization in the rat ovary, oviduct, and uterus.
MedLine Citation:
PMID:  18824635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hormone-sensitive lipase (HSL) is a key regulator of cholesterol esters metabolism. The aim of this study was to determine HSL localization in rat female reproductive organs during the ovarian cycle by IHC methods. HSL was located in the ovarian epithelium. The granulosa cells and oocytes of primordial follicles were immunonegative. In mature follicles, HSL was found in oocytes and theca and granulosa cells. However, HSL expression in theca cells and oocytes decreased during follicular atresia. Luteal cells showed HSL staining in cytoplasm during proestrus and estrus, in the nucleus during metestrus, and in cytoplasm and the nucleus during diestrus. In the tubaric ampulla, HSL was located in the epithelial cells nuclei and in the cilia during proestrus and estrus but mainly in the nucleus during metestrus and diestrus. In the isthmus, cells showed HSL immunolabeling in the nucleus and cilia during proestrus, but only in the cilia during estrus, metestrus, and diestrus. In the uterus, HSL was found in the epithelial cells nuclei. HSL-immunoreactive bands at 84, 67, 54, and 43 kDa were found in rat female reproductive organs. HSL labeling in the nucleus of epithelial and germ cells suggests an as yet unknown function for this protein, probably related to oogenesis and cell proliferation.
Authors:
María V T Lobo; Lydia Huerta; María Isabel Arenas; Rebeca Busto; Miguel Angel Lasunción; Antonia Martín-Hidalgo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-29
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  57     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-22     Completed Date:  2009-02-13     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  51-60     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Genetics, Alcalá de Henares University, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Estrus
Fallopian Tubes / cytology,  enzymology*
Female
Immunohistochemistry
Ovary / cytology,  enzymology*
Rats
Rats, Wistar
Sterol Esterase / metabolism*
Uterus / cytology,  enzymology*
Vagina / enzymology
Chemical
Reg. No./Substance:
EC 3.1.1.13/Sterol Esterase
Comments/Corrections

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