| Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes. | |
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MedLine Citation:
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PMID: 20708600 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of the present study was to investigate the effect of acute exercise on lipolysis via coordination of hormone-sensitive lipase (HSL) and scaffold proteins, i.e., perilipin A and comparative gene identification-58 (CGI-58), in rat primary adipocytes. Glycerol release was significantly elevated immediately (0h) and three hours (3h) after exercise. Both activity and localization to the pellet of HSL were significantly greater in the pellet fraction, which is included in lipid droplet associated-proteins, than in the supernatant fraction. In the pellet fraction, although neither perilipin A nor CGI-58 protein level changed, level of perilipin A/CGI-58 complex was significantly reduced, accompanied by up-regulated association of perilipin A/HSL at 0h and 3h after exercise. On the other hand, there were no changes in these molecules at 24h after exercise, despite a significant decrease in lipolysis that was observed in response to isoproterenol. These findings suggest that acute exercise enhances lipolysis up to at least 3h after exercise in a manner dependent on modification of HSL and its association with and alteration in scaffold protein. |
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Authors:
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Junetsu Ogasawara; Sachiko Nomura; Nazibur Rahman; Takuya Sakurai; Takako Kizaki; Tetsuya Izawa; Hitoshi Ishida; Shukoh Haga; Hideki Ohno |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-12 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 400 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-13 Completed Date: 2010-10-11 Revised Date: 2011-10-17 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 134-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan. junetsu@ks.kyorin-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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enzymology* Animals Carrier Proteins / biosynthesis Lipolysis* Male Phosphoproteins / biosynthesis Physical Conditioning, Animal* Rats Rats, Wistar Sterol Esterase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/CGI-58 protein, rat; 0/Carrier Proteins; 0/Phosphoproteins; 0/perilipin 1; EC 3.1.1.13/Sterol Esterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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