Document Detail

Hormone secretion stimulated by ethanol-induced cell swelling in normal rat adenohypophysial cells.
MedLine Citation:
PMID:  1905486     Owner:  NLM     Status:  MEDLINE    
Ethanol has been reported to affect endocrine functions, but its mechanism of action is unclear. To evaluate the hypothesis that cell swelling induced by ethanol permeation through the plasmalemma triggers hormone secretion, we studied the effect of ethanol on both hormone secretion and cell volume in acutely dispersed rat adenohypophysial cells under isotonic and hypertonic conditions. Isotonic ethanol caused a prompt cell swelling and an explosive secretory burst of prolactin and thyrotropin, which were proportional to the concentration of ethanol between 10 and 120 mM. The lowest effective dose of isotonic ethanol was 10 mM, which is below the plasma levels of legal intoxication (16 mM). Removal of medium Ca2+ enhanced the isotonic ethanol-induced increases in both cell volume and secretion. Hypertonic ethanol was ineffective in these effects. These data indicate that, in normal rat adenohypophysial cells, cell swelling caused by the rapid passage of ethanol through the plasmalemma is a potent mechanism for stimulating hormone secretion and this induced secretion is negatively modulated by extracellular Ca2+.
N Sato; X B Wang; M A Greer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  260     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-07-30     Completed Date:  1991-07-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E946-50     Citation Subset:  IM    
Department of Medicine, Oregon Health Sciences University, Portland 97201.
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MeSH Terms
Calcium / pharmacology
Cell Membrane / drug effects,  physiology
Cell Membrane Permeability / drug effects*
Ethanol / pharmacology*
Hypertonic Solutions
Mannitol / pharmacology
Pituitary Gland, Anterior / cytology,  drug effects,  secretion*
Prolactin / secretion*
Rats, Inbred Strains
Thyrotropin / secretion*
Grant Support
Reg. No./Substance:
0/Hypertonic Solutions; 64-17-5/Ethanol; 69-65-8/Mannitol; 7440-70-2/Calcium; 9002-62-4/Prolactin; 9002-71-5/Thyrotropin

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