| Hormone-induced co-transport with specific pharmacological properties in erythrocytes of rainbow trout, Salmo gairdneri. | |
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MedLine Citation:
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PMID: 6747848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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On the addition of isoprenaline to an isotonic suspension of red blood cells of rainbow trout (Salmo gairdneri), the cell volume increases. This increase in volume is the result of net uptake of Na+ and osmotically obligated water. Two different pathways are involved in the salt uptake. The minor component of Na+ entry (about 20%) corresponds to a Na+ uptake independent of Cl- and is inhibited by amiloride, yet is insensitive to DIDS, furosemide and niflumic acid. It could result from Na+/H+ countertransport. The major component of salt uptake is due to Na+ entry which requires Cl- as anion, and is electroneutral, independent of extracellular K+, sensitive to amiloride, DIDS, niflumic acid and furosemide, but insensitive to other loop diuretics such as piretanide or bumetanide. These characteristics, as well as the response of valinomycin-treated cells to isoprenaline and some other properties (ionic selectivity, drug sensitivity) of the anion exchange system of volume-static trout red cells, permit the definition of the nature of this Cl--dependent pathway. The findings are inconsistent with the electrically silent double antiporter model (proposed in amphibian red cells by Cala, 1980) and with the co-migration of Cl- with Na+ through parallel conductive pathways, but strongly suggest a symport mechanism. Striking differences, mainly pharmacological, exist between this NaCl co-transport and the duck red blood cell Na+/K+/2Cl- co-transport (Kregenow, 1977, 1978; McManus & Schmidt, 1978). |
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Authors:
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A Baroin; F Garcia-Romeu; T Lamarre; R Motais |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of physiology Volume: 350 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 1984 May |
Date Detail:
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Created Date: 1984-08-24 Completed Date: 1984-08-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 137-57 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives, pharmacology Animals Biological Transport / drug effects Chlorides / metabolism* Diuretics / pharmacology Erythrocyte Volume / drug effects Erythrocytes / metabolism* Isoproterenol / pharmacology Niflumic Acid / pharmacology Ouabain / pharmacology Potassium / pharmacology Salmonidae / blood* Sodium / metabolism* Trout / blood* |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Diuretics; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 4394-00-7/Niflumic Acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-23-5/Sodium; 7683-59-2/Isoproterenol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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