Document Detail

Hormonal stimulation of 3H-orotic acid incorporation into RNA by serum-free cultured hepatocytes.
MedLine Citation:
PMID:  6192940     Owner:  NLM     Status:  MEDLINE    
Insulin and dexamethasone greatly stimulate the incorporation of 3H-orotic acid into RNA. Such a stimulation is associated to an increase in the uptake of the labelled precursor into the acid soluble fraction as well as in the the specific radioactivity of the nucleoside plus nucleotide pool suggesting that hormone supplementation does not affect RNA synthesis by cultured cells. The lack of effect of insulin and dexamethasone on the level of total RNA polymerase activity in nuclei isolated from cultured hepatocytes is in line with this assumption. The hormone stimulated uptake of orotic acid is dependent on protein synthesis since it is completely abolished by cycloheximide.
G Gallo; A Voci; A Cordone; E Fugassa
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell biology international reports     Volume:  7     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  417-25     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Dexamethasone / pharmacology
Hormones / pharmacology*
Insulin / pharmacology
Liver / drug effects*,  metabolism
Orotic Acid / metabolism*
Protein Biosynthesis
RNA / biosynthesis*
Time Factors
Reg. No./Substance:
0/Hormones; 11061-68-0/Insulin; 50-02-2/Dexamethasone; 63231-63-0/RNA; 65-86-1/Orotic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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