| Hormonal stimulation of 12(R)-HETE, a cytochrome P450 arachidonic acid metabolite in the rabbit cornea. | |
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MedLine Citation:
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PMID: 2119938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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12(R)-HETE [12(R)-hydroxy-5, 8, 10, 14 eicosatetraenoic acid] is one of the major arachidonic acid metabolites produced by microsomal cytochrome P450 of the corneal epithelium. This metabolite is a potent inhibitor of Na(+)-K(+)-ATPase activity in several tissues. We investigated endogenous production of 12(R)-HETE in the rabbit corneal epithelium. Incubation of corneal epithelial sheets (prelabeled with 14C-arachidonic acid) with arginine vasopressin resulted in the production of radioactive 12(R)-HETE suggesting its formation from endogenously labeled-arachidonic acid. The maximal response was obtained with 1 microM arginine vasopressin and represents a 15-fold increase in 12(R)-HETE formation compared with that of control tissues. Stimulation of 14C-arachidonic acid release with a detergent, digitonin, also resulted in endogenous 12(R)-HETE formation. Analysis of the incubation media following digitonin treatment of prelabeled corneal epithelial sheets revealed that 12(R)-HETE production was maximal at 20 microM digitonin, a 17-fold increase over control values. This study is the first to describe hormonal and traumatic stimulation of 12(R)-HETE formation from endogenously labeled arachidonic acid in intact corneal tissues. This study demonstrates that the formation of this Na(+)-K(+)-ATPase inhibitor can be modulated by physiological and pathophysiological regulation. |
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Authors:
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K L Davis; M W Dunn; M L Schwartzman |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Current eye research Volume: 9 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-11-15 Completed Date: 1990-11-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 661-7 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, New York Medical College, Valhalla. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Animals Arachidonic Acid Arachidonic Acids / metabolism* Arginine Vasopressin / pharmacology Chromatography, High Pressure Liquid Cornea / drug effects, enzymology, metabolism* Cytochrome P-450 Enzyme System / metabolism* Digitonin / pharmacology Epithelium / drug effects, enzymology, metabolism Hydroxyeicosatetraenoic Acids / metabolism* Male Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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EY06513/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Hydroxyeicosatetraenoic Acids; 11024-24-1/Digitonin; 113-79-1/Arginine Vasopressin; 506-32-1/Arachidonic Acid; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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