Document Detail

Hormonal and metabolic regulation of human chondrosarcoma in vitro.
MedLine Citation:
PMID:  6293699     Owner:  NLM     Status:  MEDLINE    
Prostaglandin A1 has a profound inhibitory effect on uridine incorporation into RNA of normal cartilage whereas N6-monobutyryladenosine 3',5'-cyclic monophosphate is either stimulatory or without an effect. Sera from intact and growth hormone-treated hypophysectomized rats stimulate RNA synthesis but serum from untreated hypophysectomized rats does not. The present study investigated the in vitro regulation of [3H]uridine incorporation into RNA of six human chondrosarcomas to determine if malignant human chondrocytes are under similar metabolic and hormonal regulation. Prostaglandin A1 (25 micrograms/ml) markedly inhibited uridine incorporation in all six tumors (56 to 80%). N6-Monobutyryladenosine 3',5'-cyclic monophosphate (1 mM) inhibited uridine incorporation in five tumors (20 to 50%). Uridine incorporation was stimulated by growth hormone-dependent serum factors in one tumor and by growth hormone-independent serum factors in two tumors. Two tumors were more responsive to serum from growth hormone-treated hypophysectomized rats than to serum from intact rats, and one tumor was unresponsive to serum stimulation. The data indicate that: (a) prostaglandin A1 is a very potent inhibitor of RNA synthesis in human chondrosarcomas; (b) N6-monobutyryladenosine 3',5'-cyclic monophosphate affects human chondrosarcomas differently than it does normal cartilage; and (c) responses of human chondrosarcomas to serum growth factors vary among individual tumors.
W D McCumbee; J M Harrelson; H E Lebovitz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  43     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  513-6     Citation Subset:  IM    
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MeSH Terms
Bucladesine / analogs & derivatives,  pharmacology
Cell Division / drug effects
Cell Survival / drug effects
Chondrosarcoma / physiopathology*
Culture Media
Growth Hormone / pharmacology
Organ Culture Techniques
Prostaglandins A / pharmacology
Transcription, Genetic / drug effects
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Prostaglandins A; 13117-60-7/monobutyryl cyclic AMP; 14152-28-4/prostaglandin A1; 362-74-3/Bucladesine; 9002-72-6/Growth Hormone

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