Document Detail


Hormonal control of apoptotic cell death in the testis: gonadotropins and androgens as testicular cell survival factors.
MedLine Citation:
PMID:  8316250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the requirement for pituitary gonadotropins during testicular cell differentiation is well documented, the possible role of FSH and LH in regulating testicular cell survival has not been studied. Using a quantitative autoradiographic method for the detection of internucleosomal DNA fragmentation, a hallmark feature of apoptosis, the hormonal control of apoptotic cell death was studied in testicular cells collected from immature rats after hypophysectomy. After surgery, animals were treated with daily injections of 20 IU long-acting FSH agonist (FSH-CTP) or 50 IU human CG (hCG) for 2 days. Hypophysectomy decreased testis weight by 25%, but treatment with FSH-CTP or hCG prevented the effect of hypophysectomy. Testes of intact animals contained predominantly high-mol wt DNA, whereas hypophysectomy increased DNA cleavage into low-mol wt (< 15 kilobases) ladders characteristics of apoptosis. In contrast, treatment with FSH-CTP or hCG inhibited hypophysectomy-induced apoptotic DNA cleavage by 84% and 51%, respectively. Hypophysectomy-induced DNA fragmentation was found in both interstitial cells and seminiferous tubules. Similar to whole testis, treatment with FSH-CTP suppressed hypophysectomy-induced apoptosis by over 90% in seminiferous tubules and interstitial cells. In contrast, hCG treatment was less effective in preventing hypophysectomy-induced DNA cleavage (46% suppression in tubules and 77% suppression in interstitial cells). Furthermore, testosterone replacement also suppressed hypophysectomy-induced DNA fragmentation by 75% in the whole testis tissue, 64% in tubules, and 55% in interstitial cells. To further study the role of gonadotropins, intact animals were treated with a potent GnRH antagonist (Azaline B, 10 microgram/day) to decrease serum gonadotropin levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J S Tapanainen; J L Tilly; K K Vihko; A J Hsueh
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  7     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-23     Completed Date:  1993-07-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  643-50     Citation Subset:  IM    
Affiliation:
Department of Gynecology and Obstetrics, Stanford University School of Medicine, California 94305-5317.
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MeSH Terms
Descriptor/Qualifier:
Androgens / physiology*
Animals
Apoptosis / drug effects,  physiology*
Chorionic Gonadotropin / pharmacology
DNA / metabolism
Follicle Stimulating Hormone / pharmacology
Follicle Stimulating Hormone, beta Subunit
Gonadotropin-Releasing Hormone / analogs & derivatives,  antagonists & inhibitors,  pharmacology
Gonadotropins, Pituitary / physiology*
Hypophysectomy
Leydig Cells / drug effects,  metabolism
Male
Peptide Fragments / pharmacology
Rats
Rats, Sprague-Dawley
Seminiferous Tubules / drug effects,  metabolism
Testis / cytology*
Grant Support
ID/Acronym/Agency:
1-F05-TWO-4355-01/TW/FIC NIH HHS; HD-07556/HD/NICHD NIH HHS; HD-13527/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Androgens; 0/Chorionic Gonadotropin; 0/Follicle Stimulating Hormone, beta Subunit; 0/Gonadotropins, Pituitary; 0/Peptide Fragments; 134457-26-4/azaline; 33515-09-2/Gonadotropin-Releasing Hormone; 9002-68-0/Follicle Stimulating Hormone; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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