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Horizontal Distribution Of The Fecal Microbiota In Adolescents With Inflammatory Bowel Disease.
MedLine Citation:
PMID:  21788912     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKROUND AND AIMS:: The commensal microbiota of the gastrointestinal tract plays an important role in pathogenesis of inflammatory bowel disease (IBD). We examined what is the horizontal structure of the fecal microbiota in the colon in adolescents with Crohn's disease (CD), ulcerative colitis (UC) and control group. MATERIALS AND METHODS:: Fecal samples were collected in three fractions from patients with CD (n = 22), UC (n = 12) and control (n = 24) during preparation to colonoscopy. Additionally, biopsies from the colon tissue were taken. Samples were examined using a culture technique and a fluorescent in situ hybridization (FISH) method. The mucin degradation assay was carried out. RESULTS:: Quantitative composition of the microbiota was different in the consecutive three fecal fractions and in the colon tissue of the study groups but in patients from the control group the composition of microbiota in the consecutive fractions was similar. Statistical analyses showed that the total distribution of the studied bacterial taxons in the contents, in all three fecal fractions and in the colon tissue in the given disease group, as well as in the control group was characteristic for the studied patient group. Differences in species distribution among cohorts studied were highly significant (p < 0.0001). Moreover, it was showed that in the first fecal fraction and in the colon tissue samples there is no significance difference for any of the analyzed bacterial groups, both using the culture methods or FISH, but significant results were demonstrated in the II and III fractions for specific bacterial groups. The bacterial flora attached to the mucous layer in the UC group significantly more degraded mucus in comparison to the control group (p = 0.045). CONCLUSIONS:: Distribution of the microbiota in the colon is layered, what can be named horizontal distribution of the fecal flora. Only in the UC group the bacterial flora attached to the mucous layer exerts action on the mucin.
Authors:
Tomasz Gosiewski; Magdalena Strus; Krzysztof Fyderek; Kinga Kowalska-Duplaga; Andrzej Wedrychowicz; Urszula Jedynak-Wasowicz; Malgorzata Sladek; Stanislaw Pieczarkowski; Pawel Adamski; Piotr Heczko
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-22
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  -     ISSN:  1536-4801     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
* Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Str., 31-121 Cracow, Poland † Department of Pediatrics, Gastroenterology and Nutrition, Polish-American Children's Hospital, Jagiellonian University Medical College, 265 Wielicka Str., 30-663 Cracow, Poland ‡ Department of Pediatrics, Polish-American Children's Hospital, Jagiellonian University Medical College, 265 Wielicka Str., 30-663 Cracow, Poland § Institute of Nature Conservation, Polish Academy of Sciences, 33 Mickiewicza Ave., 31-120 Cracow, Poland.
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